Browse

Effect of serum-derived albumin scaffold and canine adipose tissue-derived mesenchymal stem cells on osteogenesis in canine segmental bone defect model
개의 결손골 모델에서 자가혈청유래 알부민 스캐폴드와 개 지방유래 간엽줄기세포의 골 형성 촉진

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
윤대영
Advisor
권오경
Major
수의과대학 수의학과
Issue Date
2014-02
Publisher
서울대학교 대학원
Keywords
Adipose-derived mesenchymal stem cellsBone defectSerum-derived albumin scaffold
Description
학위논문 (석사)-- 서울대학교 대학원 : 수의학과, 2014. 2. 권오경.
Abstract
Composite synthetic grafts with progenitor cells instead of auto or allografts offer an alternative approach for the fracture repair. The aim of this study was to evaluate osteogenesis of autologous serum-derived albumin (ASA) scaffold seeded with canine adipose tissue-derived mesenchymal stem cells (Ad-MSCs) in canine segmental bone defect model. ASA scaffold was prepared with canine serum by cross-linking and freeze-drying procedures. Beta-tricalcium phosphate (β-TCP) was mixed at cross-linking stage of the procedures. Ad-MSCs were seeded into scaffold and incubated for a day before implantation. The grafts were harvested 16 weeks after implantation for histological analysis. Dogs were divided into five groups
control, ASA scaffolds with and without Ad-MSCs, ASA scaffolds including β-TCP with and without Ad-MSCs. The group of ASA scaffold with Ad-MSCs had larger radiopaque area on radiographs than the groups with β-TCP and more bone formation in histological finding than other groups (p < 0.05). It was suggested that Ad-MSCs seeded into ASA scaffold enhanced osteogenesis in bone defect model but β-TCP in the ASA scaffold prevented the penetration of the cells related to bone healing. However, more time is required to verify whether fibrous tissues in the middle of the defects could be converted to osteoprogenitor cells.
Language
English
URI
https://hdl.handle.net/10371/133708
Files in This Item:
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Theses (Master's Degree_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse