S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Theses (Master's Degree_수의학과)
Hair Growth Promoting Effect of Dermal Papilla Like Tissues from Canine Adipose-Derived Mesenchymal Stem Cells in Athymic Nude Mice
무흉선 마우스에서 개의 지방 중간엽 줄기세포 유래 진피유두 유사조직의 발모 촉진 효과
- 수의과대학 수의학과
- Issue Date
- 서울대학교 대학원
- adipose-derived mesenchymal stem cells; athymic nude mouse; dermal papilla like tissues; hair regeneration; vascular endothelial growth factor
- 학위논문 (석사)-- 서울대학교 대학원 : 수의학과, 2016. 8. 김완희.
- The purpose of this study was to investigate the protein expression pattern and the in vivo trichogenicity of dermal papilla like tissues (DPLTs) which made from canine adipose-derived mesenchymal stem cells (ASCs) in athymic nude mice. Canine ASCs were isolated and cultured from adipose tissue, and differentiation was induced by culturing ASCs in dermal papilla forming media. DPLTs were embedded in collagen gel and their structural characteristics and protein expression were evaluated by hematoxylin and eosin stain and immunohistochemistry. Athymic nude mice were divided into two groups (control and DPLTs groups) and DPLTs were injected in skin wounds of mice in the DPLTs group. The trichogenicity of DPLTs was assessed by gross and histological evaluation for 30 days. The fate and the growth factor-secretion effect of DPLTs were evaluated by immunohistochemistry and Western blotting. DPLTs have a compact aggregated structure, form extracellular matrix and highly express the protein specific for dermal papillae, including ALP and versican. New hair follicle formation was remarkable in nude mice of the DPLTs group in gross findings and H&E stain. Vascularization was increased in the DPLTs group, which was the effect of vascular endothelial growth factor secreted by DPLTs in vitro and in vivo. These data suggest that engineered canine DPLTs have characteristics of dermal papillae and have a positive effect on hair regeneration by secreting growth factors.