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Hepatic differentiation of hunan adipose tissue-derived mesenchymal stem cells and adverse effects of arsanilic acid and acetaminophen during in vitro hepatic developmental stage
인체 지방유래 중간엽 줄기세포의 간세포 분화 및 비소와 아세트아미노펜의 간세포 분화에 미치는 영향 평가

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Authors
권미정
Advisor
조제열
Major
수의과대학 수의학과
Issue Date
2017-02
Publisher
서울대학교 대학원
Keywords
Human adipose tissue-derived mesenchymal stem cellHepatocyte-like cellHepatotoxicityArsanilic AcidAcetaminophen
Description
학위논문 (석사)-- 서울대학교 대학원 : 수의학과, 2017. 2. 조제열.
Abstract
In the present study, the hepatocyte-like cells (HLCs) were differentiated from human adipose tissue-derived mesenchymal stem cells (AT-MSCs). The hepatic differentiation was confirmed by increases in hepatic proteins or genes, the cytochrome P450 (CYP) activities, albumin secretion and glycogen storage. To determine the developmental toxic effect of Arsanilic acid (Ars) and acetaminophen (AAP) on the hepatic development, the differentiating cells were treated with the test chemicals (below IC12.5)from day 4 to day 13. The enzymatic activities of lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) did not significantly differ in response to Ars treatment. AAP treatment increased the activities of all enzymes in a dose-dependent manner, significantly at concentrations of 2.5 and 5mM AAP. On the expressions of hepatic genes for Ars, the expressions were significantly inhibited by more than 0.5 mM for Albumin (ALB) but only 2.5 mM for α-feto protein (AFP). In the AAP-treated group, the expressions of ALB and AFP were significantly decreased at the concentrations exceeding 0.625 mM. The activities of CYP3A4 were not changed by both treatments. The activities of CYP1A2 were increased by AAP, whereas it was decreased by Ars treatment. In conclusion, AAP could cause serious adverse effects during the hepatic development as compared to Ars.
Language
English
URI
https://hdl.handle.net/10371/133769
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Theses (Master's Degree_수의학과)
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