Different effects of Lace1 on white and brown adipogenic differentiation in vitro.

Abstract

The mammalian homologue of yeast Afg1, lactation elevated 1 (LACE1), has been known that regulates mitochondrial protein homeostasis, has an interaction with p53 and indicates pro-apoptotic activity in HEK293 cells. Functions of LACE1 in other cell types are poorly understood. Thus, We measured the expression of LACE1 throughout adipogenic differentiation. Also, we knocked down the expression of LACE1 in 3T3-L1 white pre-adipocytes and HIB1B brown pre-adipocytes, and then induced differentiation. In present study, we report that LACE1 increases during white and brown adipogenesis using in vitro system. Knock-down of LACE1 leads to increases of mature white adipogenic marker (aP2), a main adipogenic transcription factors (C/ebpα, Pparγ) and exhibits down-regulations of brown adipocyte markers, mitochondrial biogenesis markers and mtDNA encoded complex Ⅳ subunits. Also, we observe that ablation of LACE1 induces reduction of p53 expression. We conclude that LACE1 inhibits white adipogenic differentiation and has a function in brown adipogenic differentiation.