S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Theses (Master's Degree_수의학과)
Role of CCL4 and CCR5 during osteoclastogenesis of murine bone marrow-derived monocytes
마우스 골수 유래 단핵세포로부터 파골세포로의 분화 과정에서 MEK과 JNK 신호에 의한 CCR5 수용체 조절 및 역할
- 수의과대학 수의학과
- Issue Date
- 서울대학교 대학원
- 학위논문 (석사)-- 서울대학교 대학원 : 수의학과, 2017. 2. 조제열.
- Chemokine CCL4 (MIP-1β) is released from osteoblast cells to restore the homeostasis of hematopoietic stem cells during the activation of bone marrow. In this study, the function of CCL4 during osteoclastogenesis was investigated. CCL4 promoted the migration and viability of pre-osteoclast cells. However, CCL4 had no direct effect on the receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis in mouse pre-osteoclast cells. During osteoclastogenesis, the expression of CCR5, the CCL4 receptor, was rapidly reduced by RANKL treatment. CCR5 down-regulation by RANKL was mediated by MEK and JNK in pre-osteoclast cells and played a role in osteoclastogenesis. These results suggest that the chemoattractant effect and viability of CCL4 is involved in recruiting pre-osteoclasts but is diminished later its effect on osteoclastogenesis by the reduction of CCR5 when RANKL is prevalent.