Matrix-assisted variable wavelength laser desorption ionization of peptide
다양한 파장에서 펩타이드의 매트릭스 보조 레이저 탈착/이온화
- 자연과학대학 화학부
- Issue Date
- 서울대학교 대학원
- plume temperature; in-source decay; post-source decay; matrix absorption coefficient; expansion cooling
- 학위논문 (석사)-- 서울대학교 대학원 : 화학부 물리화학 전공, 2013. 2. 김명수.
- Product ion yields in the in- and post-source decays of three peptide ions, [Y5X + H]+ (X = Y (tyrosine), K (lysine), and R (arginine)), generated by matrix-assisted laser desorption ionization (MALDI) were measured at six wavelengths, 307, 317, 327, 337, 347, and 357 nm, using α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB) as the matrices. The temperatures of the early and late plumes generated by MALDI were estimated via kinetic analysis of the product ion yield data. For both matrices, the temperature drop (∆T), i.e. the difference in the temperature between the early and late plumes, displayed negative correlation with the absorption coefficient. This was in agreement with the previous reasoning that deeper laser penetration and larger amount of material ablation arising from smaller absorption coefficient would result in larger extent of expansion cooling. The results support the postulation of the expansion cooling occurring in the plume presented previously.