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Liquid extraction surface analysis coupled with capillary electrophoresis to determine pesticides on apple skin : 사과 표면의 농약 검출을 위한 액체 추출 표면 분석법과 연동된 모세관 전기영동법
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 정두수 | - |
dc.contributor.author | 성인혜 | - |
dc.date.accessioned | 2017-07-27T02:18:41Z | - |
dc.date.available | 2017-07-27T02:18:41Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.other | 000000016885 | - |
dc.identifier.uri | https://hdl.handle.net/10371/134896 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 화학부(분석화학전공), 2014. 2. 정두수. | - |
dc.description.abstract | A surface-sampling technique of liquid extraction surface analysis (LESA) was coupled with capillary electrophoresis (CE) to determine organophosphorous pesticides, including glufosinate-ammonium, aminomethylphosphonic acid, and glyphosate on the external surface of a fruit such as an apple. Without any sample pretreatment, the analytes sprayed on the surface of a half apple were directly extracted into a liquid microjunction formed by dispensing the extractant from the inlet tip of a separation capillary. The extraction efficiency was enhanced by repeating the steps of dispensing and aspirating the extractant drop. After extraction, the analytes were derivatized in-capillary with a fluorophore 4-flouro-7-nitro-2,1,3-benzoxadiazole and analyzed with CE-laser induced fluorescence (LIF) detection. The developed LESA-CE/LIF carries out quantitative analysis in a convenient and rapid manner, which is made possible since the extraction and separation of analytes can be performed in-line. The limits of detection for glufosinate-ammonium, aminomethylphosphonic acid, and glyphosate with LESA-CE/LIF were 2.5, 1, and 10 ppb, respectively, which are at least 20 times lower than the tolerance established by the U.S. Environmental Protection Agency. Thus, we demonstrated that LESA-CE is a quite sensitive and convenient method to determine analytes on sample surfaces avoiding the dilution problem from sample pretreatment procedures including homogenization of bulk samples. | - |
dc.description.tableofcontents | ABSTRACT i
CONTENTS iii LIST OF FIGURES AND TABLES iv 1 INTRODUCTION 1 2 EXPERIMENTAL 4 2.1 Reagents 4 2.2 Apparatus 5 2.3 Sample preparation 6 2.4 LESA 6 2.5 CE-LIF 9 2.6 Solid phase extraction-CE/LIF 11 3 RESULTS AND DISCUSSION 12 3.1 Number of extraction cycles 12 3.2 Optimization of in-capillary derivatization 12 3.3 Analytical performance of LESA-CE/LIF 15 3.4 LESA-CE from an unspiked apple surface 18 4. CONCLUSIONS 20 REFERENCES 21 ABSTRACT IN KOREAN | - |
dc.format | application/pdf | - |
dc.format.extent | 629661 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Liquid extraction surface analysis | - |
dc.subject | Capillary electrophoresis | - |
dc.subject | Organophosphorous pesticides | - |
dc.subject | Pesticide residue analysis | - |
dc.subject.ddc | 540 | - |
dc.title | Liquid extraction surface analysis coupled with capillary electrophoresis to determine pesticides on apple skin | - |
dc.title.alternative | 사과 표면의 농약 검출을 위한 액체 추출 표면 분석법과 연동된 모세관 전기영동법 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | In Hye Sung | - |
dc.description.degree | Master | - |
dc.citation.pages | iv, 24 | - |
dc.contributor.affiliation | 자연과학대학 화학부 | - |
dc.date.awarded | 2014-02 | - |
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