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Astrocytic expressions of phosphorylated Akt, GSK3beta and CREB following an excitotoxic lesion in the mouse hippocampus

Cited 27 time in Web of Science Cited 25 time in Scopus
Authors
Kim, Dong Woon; Lee, Jung Hoon; Park, Sung Kyung; Yang, Woo-Mi; Jeon, Gye Sun; Lee, Young Ho; Chung, Chun Kee; Cho, Sa Sun
Issue Date
2007-04-10
Publisher
Springer
Citation
Neurochem Res. 2007 Sep;32(9):1460-8. Epub 2007 Apr 7.
Keywords
AnimalsAstrocytes/*metabolismBlotting, WesternCyclic AMP Response Element-Binding Protein/*biosynthesisGene Expression ProfilingGlycogen Synthase Kinase 3/*biosynthesisHippocampus/drug effectsKainic Acid/toxicityMaleMiceMice, Inbred ICRPhosphorylationProto-Oncogene Proteins c-akt/*biosynthesis
Abstract
Glycogen synthase kinase 3beta (GSK3beta) is believed to play important roles in the regulation of synaptic plasticity, cell survival and circadian rhythms in the mature CNS. However, although several studies have been focused on the GSK3beta, little is known about GSK3beta changes in glial cells under neuropathological conditions. In this study, we evaluated the expressions of molecules associated with the GSK3beta signaling pathway, following the induction of an excitotoxic lesion in mouse brain by kainic acid (KA) injection, which caused pyramidal cell degeneration in the hippocampal CA3 region. In injured hippocampi, Ser47-Akt (protein kinase B, PKB) phosphorylation increased from 4 h until 1 day post-injection (PI). Ser9-GSK3beta and Ser133-cAMP responsive element-binding protein (CREB) phosphorylations showed similar spatiotemporal patterns in hippocampi at 1 day until 3 days PI. Double immunohistochemistry also showed that these phosphorylated forms of Akt, GSK3beta and CREB were expressed in astrocytes. For the first time, our data demonstrate the injury-induced astrocytic changes in the levels of phosphorylation of Akt, -GSK3beta and -CREB in vivo, which may reflect mechanisms of glial cells protection or adaptive response to damage.
ISSN
0364-3190 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17417726

https://hdl.handle.net/10371/13616
DOI
https://doi.org/10.1007/s11064-007-9332-y
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College of Medicine/School of Medicine (의과대학/대학원)Neurosurgery (신경외과학전공)Journal Papers (저널논문_신경외과학전공)
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