S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Chemical and Biological Engineering (화학생물공학부) Theses (Master's Degree_화학생물공학부)
Efficient Synthesis of Peptide Conjugated Perylene Diimide Bola-Amphiphile using 2-Chlorotrityl Chloride Resin and its Application to Photodynamic Therapy
2-CTC 수지를 이용한 효과적인 펩타이드-퍼릴렌의 합성 및 광역학치료로의 응용
- 공과대학 화학생물공학부
- Issue Date
- 서울대학교 대학원
- perylene-3; 4; 9; 10-tetracarboxylic dianhydride; solid phase peptide synthesis; RGD sequence; photodynamic therapy
- 학위논문 (석사)-- 서울대학교 대학원 공과대학 화학생물공학부, 2017. 8. 이윤식.
- Perylene-3,4,9,10-tetra carboxylic diimide (PDI) has remarkable features as a photosensitizer in Photodynamic Therapy. However, PDIs poor solubility in aqueous condition limits its use. So it is commonly functionalized by various substituents on imide positions where two nitrogen groups comprised with imide moiety. However, there has been a technical limitation to conjugate them with proper amine gorups such as amino acids or peptides having pi-electron system. To overcome this problem, solid-phase peptide synthesis (SPPS) method has been utilized to conjugate peptides having diverse pi-electron functional molecules. But still other problems appeared such as low yield with harsh reaction condition. Therefore, a new method was designed to overcome such disadvantages of conventional SPPS method. For this, reaction times, loading level, and solvent system were optimized. In this research, RGD sequence was conjugated as a substituent on imide position. The RGD sequence is well known as a cell attachment component. Thus, we could develope very useful materials which has both strong cell adhesion ability and photosensitizer for photodynamic therapy.