Enzymatic production of functional indigestible isomaltooligosaccharides using glucansucrases from Leuconostoc mesenteroides B-512FMCM and L. mesenteroides NRRL B-1355CF10

Abstract

The novel indigestible isomaltooligosaccharides (IDMOS) was synthesized by combination of two glucansucrases from Leuconostoc mesenteroides B-512FMCM (DSase512) and L. mesenteroides NRRL B-1355CF10 (GSase1355) from Kimchi, Korean traditional fermented food. By acceptor reaction, glucose (donor) was covalently bonded to maltose (acceptor) with α-glycosidic linkages. Conditions for synthesizing the IDMOS were tested by setting 4 variables. The synthesized the IDMOS was analyzed by TLC (thin layer chromatography) and HPLC (high-performance liquid chromatography). As a result, 85% of the IDMOS was remained after treatment with intestinal enzymes (α-amylase and amyloglucosidase). Using yeast beads, simple sugars in the IDMOS were selectively removed. One function of the IDMOS was prevention of insoluble glucan (mutan) formation as prebiotics. When 10 % (w/v) of the IDMOS was treated at 5 % (w/v) sucrose concentration, the amount of mutan formation was reduced about 50%. The IDMOS was also used as a carbon source for growth of Bifidobacteria. In IDMOS added media (MRS-IDMOS), Bifidobacteria were grown well and they used the IDMOS selectively. Especially, the Bifidobacterium adolescentis strain consumed the IDMOS the most by verifying TLC analysis. And as Bifidobacteria growing, growth of food pathogen like Salmonella was inhibited. Synthesized the IDMOS by GRAS (generally recognized as safe) bacteria and having bifidogenic effect is expected matter for food industry.