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Overexpression of OsTZF8, a CCCH-Tandem Zinc finger, increases drought tolerance in rice : CCCH –Tandem Zinc finger인 OsTZF8의 과발현 벼의 가뭄저항성 증가에 대한 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김주곤 | - |
dc.contributor.author | 성소윤 | - |
dc.date.accessioned | 2017-10-31T07:43:44Z | - |
dc.date.available | 2017-10-31T07:43:44Z | - |
dc.date.issued | 2017-08 | - |
dc.identifier.other | 000000146038 | - |
dc.identifier.uri | https://hdl.handle.net/10371/137478 | - |
dc.description | 학위논문 (석사)-- 서울대학교 국제농업기술대학원 국제농업기술학과, 2017. 8. 김주곤. | - |
dc.description.abstract | Tandem CCCH zinc finger proteins (TZFs), members of the zinc finger protein family, have been known to participate as post-transcriptional regulators of gene expression in eukaryotes. However, the function of TZF as a drought stress-related gene remains unclear. Here, we show that the OsTZF8, a rice gene for tandem CCCH zinc finger protein, is induced by abiotic stress and its transgenic rice plants overexpressing OsTZF8 increases drought tolerance. Gene expression analysis revealed that OsTZF8 is specifically expressed in embryo, but not in vegetative organs. In field evaluation, grain yield and seed size were higher in the transgenic plants with constitutive overexpression than non-transgenic plants under drought conditions. OsTZF8 not only localizes in the nucleus, but also co-localizes with both processing bodies and stress granules, two messenger ribo-nucleoprotein complexes which are known to activate by forming cytoplasmic foci under stress conditions. Taken together, these results suggest that OsTZF8 participates in RNA turnover in processing bodies and stress granules regulating drought stress in rice. | - |
dc.description.tableofcontents | INTRODUCTION 1
MATERIALS AND METHODS. 3 1. Plant growth for abiotic stress and stress treatment. 3 2. Plant growth for visual test and drought stress tolerance test 3 3. RNA extraction. 4 4. cDNA synthesis.. 5 5. Quantitative real-time PCR. 5 6. Agronomic trait analysis. 6 7. Subcellular localization using rice protoplast. 6 RESULTS 8 1. Phylogenetic tree of Zinc finger in the Oryza sativa. 8 2. Expression patterns of OsTZF8. 8 3. Drought stress tolerance visual test of PGD1:OsTZF8 and RCc3:OsTZF8. 10 4. Agronomic trait of Overexpression transgenic plant of OsTZF8 and non-transgenic plant grown under normal and drought condition in the field.. 11 5. Phenotypic characterization of grains in NT and PGD1:OsTZF8 transgenic plant. 11 6. Subcellular localization of OsTZF8 12 7. Evaluation of genes regulated by OsTZF8 by RNA-seq data 13 DISCUSSION 42 REFERENCE.. 45 ABSTRACT IN KOREAN.. 48 | - |
dc.format | application/pdf | - |
dc.format.extent | 2912266 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 국제농업기술대학원 | - |
dc.subject | Tandem CCCH zinc finger | - |
dc.subject | Rice | - |
dc.subject | Drought tolerance | - |
dc.subject | Processing bodies (PB) | - |
dc.subject | Stress granules (SG) | - |
dc.subject.ddc | 631 | - |
dc.title | Overexpression of OsTZF8, a CCCH-Tandem Zinc finger, increases drought tolerance in rice | - |
dc.title.alternative | CCCH –Tandem Zinc finger인 OsTZF8의 과발현 벼의 가뭄저항성 증가에 대한 연구 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 국제농업기술대학원 국제농업기술학과 | - |
dc.date.awarded | 2017-08 | - |
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