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Insecticidal Activities of Plant Essential Oil Compounds Showing Juvenile Hormone Agonist or Antagonist Activity : 식물체 정유 화합물의 곤충 유충호르몬 교란물질 탐색 및 살충활성 검정
DC Field | Value | Language |
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dc.contributor.advisor | 제연호 | - |
dc.contributor.author | 박동환 | - |
dc.date.accessioned | 2017-10-31T07:51:38Z | - |
dc.date.available | 2017-10-31T07:51:38Z | - |
dc.date.issued | 2017-08 | - |
dc.identifier.other | 000000145427 | - |
dc.identifier.uri | https://hdl.handle.net/10371/137580 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 농업생명과학대학 농생명공학부, 2017. 8. 제연호. | - |
dc.description.abstract | Pests cause huge economic damages not only by harming crops or livestock production but also transmitting vector borne diseases like malaria, yellow fever and dengue fever. To control pests, chemical insecticides like organophosphates, carbamates, pyrethroids have been commonly used. However, use of chemical insecticides faced several limit such as environmental threat or resistance. Insect growth regulators (IGRs) are attractive alternative pesticides due to their low environmental toxicity and high specificity. Plant essential oils have been reported to show repellent, insecticidal and growth-reducing effect on many insect herbivores. Recently, it has been reported that plants synthesize secondary metabolites regulating insect juvenile hormone (JH) receptor complex as a part of their defense mechanisms. In this study, 195 plant essential oil compounds were tested for their JH agonist and antagonist activities using a yeast two-hybrid system transformed with the Aedes aegypti JH receptor as a reporter system. Among them, 17 compound that showing high JH agonist (JHA) or antagonist (JHAN) activities were identified. They were grouped into 4 groups by their structural similarity. Their insecticidal activity and nematicidal activity were tested against Aedes albopictus, Plutella xylostella, Plodia interpunctella, Laodelphax striatellus and Bursaphelenchus xylophilus. Against A, albopictus 3rd instar larvae, 5 Plant essential oil compounds showed over 70% mortality at 10 ppm. Against 3rd instar larvae of P. xylostella, undecyl aldehyde indicated over 80% mortality at 200ppm. Against P. interpunctella, nerolidol showed over 80% mortality against 2nd instar larvae and undecyl acetate showed reduced egg hatching rate. Also benzyl sulfide, benzyl disulfide, benzyl trisulde and dodecyl aldehyde showed nematicidal activity against B. xylophilus. These results could provide insights on the plant-insect coevolution and may be useful for the development of insect specific and safe pesticides. | - |
dc.description.tableofcontents | ABSTRACT I
TABLE OF CONTENTS III LIST OF TABLES V LIST OF FIGURES VI INTRODUCTION 1 LITERATURE REVIEW 4 1. Plant essential oils as insecticide 4 2. Juvenile hormone 5 3. Insect growth regulator (IGR) 7 A. Juvenile hormone agonists 8 B. Juvenile hormone antagonists 9 C. Ecdysone agonist 10 D. Chitin synthesis inhibitor 12 METERIAL AND METHODS 14 1. Plant essential oil compounds 14 2. Insects 14 3. Yeast two-hybrid binding test using the β-galactosidase assays 18 4. Growth inhibition test 19 5. Insect dipping bioassay 19 6. Mosquito fumigation bioassay 20 7. Indian meal moth egg bioassay 21 RESULTS 22 1. JHA and JHAN activities of plant essential oil compounds 22 2. Insecticidal activity of plant essential oil compounds 31 DISCUSSION 40 LITERATURES CITED 44 ABSTRACT ON KOREAN 55 | - |
dc.format | application/pdf | - |
dc.format.extent | 1534146 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Insect growth regulator | - |
dc.subject | Plant essential oils | - |
dc.subject | Juvenile hormone agonist | - |
dc.subject | Juvenile hormone antagonist | - |
dc.subject.ddc | 630 | - |
dc.title | Insecticidal Activities of Plant Essential Oil Compounds Showing Juvenile Hormone Agonist or Antagonist Activity | - |
dc.title.alternative | 식물체 정유 화합물의 곤충 유충호르몬 교란물질 탐색 및 살충활성 검정 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Dong Hwan Park | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 농업생명과학대학 농생명공학부 | - |
dc.date.awarded | 2017-08 | - |
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