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Insights into the genomic characterization and pathogenicity
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김희발 | - |
dc.contributor.author | 구현진 | - |
dc.date.accessioned | 2017-10-31T07:51:43Z | - |
dc.date.available | 2017-10-31T07:51:43Z | - |
dc.date.issued | 2017-08 | - |
dc.identifier.other | 000000145314 | - |
dc.identifier.uri | https://hdl.handle.net/10371/137581 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 농업생명과학대학 농생명공학부, 2017. 8. 김희발. | - |
dc.description.abstract | In these studies, genome-wide studies were conducted to have insights into the genomic characterization and pathogenesis. Although many researchers have tried to focus on the pathogenesis interaction with living organisms, little genomic research has been performed into the mechanism of pathogenesis leading to the disease. Therefore, to extend comprehensive understanding at the genomic level, I conducted comparative analysis and genome-wide association study.
In chapter 2, I performed a comparative genomic analysis with the complete genomes of B. cereus strains which is well known as a gastrointestinal pathogen that induces food poisoning. Based on the result of comparative analysis, I could identify the virulence factors playing a crucial role in pathogenesis, positively selected genes and strain-specific genes of FORC_013. In chapter 3, pan-genome analysis was conducted using 20 Shigella complete genomes available on the NCBI genome database. I found not only the overall genetic contents in Shigella spp. but also newly obtained genes through horizontal gene transfer. And these species are defined as monophyletic groups using the phylogenetic tree based on the core gene clustering and ANI analysis. Genes under positive selection account for very low rate in the core genome. In chapter 4, genome-wide association studies on fusarium wilt resistance in radish (Raphanus sativus) was conducted to identify the relationship between phenotypic and genotypic data. I found the significant loci responsible for leading fusarium disease. Through these studies, I could not only understand about genetic features in living organisms but also provide a comprehensive insight into further studies regarding pathogenesis mechanism at genomic level. | - |
dc.description.tableofcontents | Chapter 1. Literature Review 1
1.1 Comparative genomic analysis 2 1.2 Genome-wide association study 7 Chapter 2. Comparative genomic analysis reveals genetic features related to the virulence of Bacillus cereus FORC_013 10 2.1 Abstract 11 2.2 Introduction 12 2.3 Materials and Methods 14 2.4 Results and Discussion 20 2.5 Conclusions 34 Chapter 3. Pan-genome analysis revealed the core genome of Shigella spp 35 3.1 Abstract 36 3.2 Introduction 37 3.3 Materials and Methods 39 3.4 Results and Discussion 42 Chapter 4. Genome-wide association studies on Fusarium wilt resistance in Raphanus sativus using genotyping-by-sequencing approach 52 4.1 Abstract 53 4.2 Introduction 54 4.3 Materials and Methods 59 4.4 Results 66 4.5 Discussion 77 References 84 국문초록 97 | - |
dc.format | application/pdf | - |
dc.format.extent | 6326591 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | comparative genomic analysis | - |
dc.subject | pan-genome analysis | - |
dc.subject | genome-wide association study | - |
dc.subject | pathogenicity | - |
dc.subject.ddc | 630 | - |
dc.title | Insights into the genomic characterization and pathogenicity | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Hyunjin Koo | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 농업생명과학대학 농생명공학부 | - |
dc.date.awarded | 2017-08 | - |
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