S-Space Graduate School of Convergence Science and Technology (융합과학기술대학원) Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과) Theses (Master's Degree_분자의학 및 바이오제약학과)
Screening of Tumor Endothelial Cell Specific MarkerDoppelfor Prediction of Anti-Cancer Effect
종양내피 세포 특이적 마커인 도펠 단백질의 항암 효과 예측을 위한 스크리닝
- 융합과학기술대학원 분자의학 및 바이오제약학과
- Issue Date
- 서울대학교 융합과학기술대학원
- 학위논문 (석사)-- 서울대학교 융합과학기술대학원 분자의학 및 바이오제약학과, 2017. 8. 변영로.
- DoppelProtein is a tumor endothelial cell (TEC) specific marker which has a unique mechanism. It is known to interact with VEGFR 2 on the surface of the tumor endothelial cells specifically which inhibit the internalization of the VEGFR 2. This phenomenon induces uncontrolled angiogenesis in tumor site that leads to tumor growth. Herein, this study screens out theDoppelprotein by different tumor types which hypothesize thatDoppelprotein could be a possible biomarker and target site for further anti-cancer studies. In order to overcome the conventional anti-angiogenic drugs limitation, non-specificity and toxicity, using 'Doppel' protein will be an efficient and novel approach for anti-angiogenic drugs for anti-cancer effect. This study used immunohistochemistry, Western blot, and flow cytometry analysis to confirm the hypothesis. Indeed, the expression levels of this protein differ by the different types of tumor, and this characteristic can be distinguishable for further analysis. The protein level difference gives the possibility of using the 'Doppel' protein as a target site forDoppelantibody and biomarker for the effective therapeutics.