A Study of MnxZn1-xFe2O4 Superparamagnetic Iron Oxide Nanoparticles and its Therapeutic Efficacy for Magnetic Nanoparticle Hyperthermia Applications

Abstract

Magnetic nanoparticles are nano-sized (less than 1 micro meter in diameter) material including iron, cobalt, and nickel. The physical & chemical properties, behavior, characteristics of magnetic nanoparticles largely depends on the synthesis method and chemical structure. In this study, we have synthesized iron oxide nanoparticles with controlled doping level of manganese and zinc, and also characterized the magnetic properties of these nanoparticles. We found that Mn0.5Zn0.5Fe2O4 nanoparticle exhibits highest AC heating characteristics at 30~370 kHz frequency and 80~160 Oe magnetic field strengths. Surface modification of Mn0.5Zn0.5Fe2O4 were successfully done with methoxy-PEG-silane, confirmed with infrared spectroscopy and dynamic light scattering. Moreover, we investigated the in vitro cytotoxic effect and qualitative uptake behavior of superparamagnetic nanoparticle on several kinds of glioblastoma cells. However, to consider the biologically and physiologically safe range of magnetic field, we fixed the frequency with 99.0 kHz and the magnetic field with 140 Oe. in vivo magnetic nanoparticle hyperthermia study was done with subcutaneous mouse tumor models, indicating that the magnetic hyperthermia can help to resist the tumor growth and tumor survival. However, magnetic nanoparticle hyperthermia therapy cannot completely cured the tumor, but the hyperthermia treatment inhibited the tumor growth rate.