Functional Study of YjfN as an Activator of DegP Protease
단백질 분해효소 DegP의 활성화 기작과 관련된 단백질 YjfN의 기능 연구
- 자연과학대학 화학부
- Issue Date
- 서울대학교 대학원
- proteolysis; stress response; suicide activiator; protein quality control; YjfN; DegP; HtrA
- 학위논문 (석사)-- 서울대학교 대학원 자연과학대학 화학부, 2017. 8. 김석희.
- DegP protease is a major constituent of protein quality control in the periplasm of Gram-negative bacteria. DegP performs regulated proteolysis of misfolded or aggregated proteins, and the function of DegP is essential for the survival of Gram-negative bacteria at high temperature. DegP shows distinct features of activity regulation compared to the cytoplasmic proteases, and it is still unknown that whether any native proteins can regulate DegP activity. In this study, we report the functions of YjfN, a previously unknown periplasmic protein. We found that it functions as a substrate of DegP. Interestingly, during its degradation, YjfN induces activation of DegP in trans. Various features of YjfN indicate that YjfN activates DegP to relieve envelope stress. To our knowledge, this is the first protein which works as a suicide activator. Our findings suggest another possible strategy of enzyme activity regulation.