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Sugary Endosperm is Modulated by Starch Branching Enzyme IIa in Rice (Oryza sativa L.)

Cited 12 time in Web of Science Cited 12 time in Scopus
Authors
Lee, Yunjoo; Choi, Min-Seon; Lee, Gileung; Jang, Su; Yoon, Mi-Ra; Kim, Backki; Piao, Rihua; Woo, Mi-Ok; Chin, Joong Hyoun; Koh, Hee-Jong
Issue Date
2017-07-20
Publisher
Springer Open
Citation
Rice, 10(1):33
Keywords
Map-based cloningOsBEIIaOsISA1RiceSugary-hSugary endosperm
Abstract
Abstract

Background
Starch biosynthesis is one of the most important pathways that determine both grain quality and yield in rice (Oryza sativa L.). Sugary endosperm, sugary-1 (sug-1), is a mutant trait for starch biosynthesis. Rice plants carrying sug-1 produce grains that accumulate water-soluble carbohydrates instead of starch, even after maturity. Although this trait enhances the diversity of grain quality, sugary endosperm rice has hardly been commercialized due to the severely wrinkled grains and subsequent problems in milling. This study was conducted to identify the genes responsible for the sug-h phenotype through a map-based cloning technology.

Results
We induced a mild sugary mutant, sugary-h (sug-h) through the chemical mutagenesis on the Korean japonica cultivar Hwacheong. Grains of the sug-h mutant were translucent and amber-colored, and the endosperm appeared less wrinkled than sug-1, whereas the soluble sugar content was fairly high. These characteristics confer greater marketability to the sug-h mutant. Genetic analyses indicated that the sug-h mutant phenotype was controlled by a complementary interaction of two recessive genes, Isoamylase1 (OsISA1), which was reported previously, and Starch branching enzyme IIa (OsBEIIa), which was newly identified in this study. Complementation tests indicated that OsBEIIa regulated the properties of sugary endosperm.

Conclusions
Complementary interactions between the starch biosynthesis genes OsISA1 and OsBEIIa determine the mild sugary endosperm mutant, sugary-h, in rice. Our finding may facilitate the breeding of sugaryendosperm rice for commercial benefit.
ISSN
1939-8433
Language
English
URI
https://hdl.handle.net/10371/138329
DOI
https://doi.org/10.1186/s12284-017-0172-3
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Plant Science (식물생산과학부)Journal Papers (저널논문_식물생산과학부)
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