S-Space College of Natural Sciences (자연과학대학) Dept. of Physics and Astronomy (물리·천문학부) Physics (물리학전공) Journal Papers (저널논문_물리학전공)
Accelerated super-resolution imaging with FRET-PAINT
- Lee, Jongjin; Park, Sangjun; Kang, Wooyoung; Hohng, Sungchul
- Issue Date
- BioMed Central
- Molecular Brain, 10(1):63
- FRET-PAINT; DNA-PAINT; super-resolution microscopy; SMLM; single-molecule localization microscopy,; FRET; fluorescence resonance energy transfer
- Super-resolution fluorescence microscopy in the current form is hard to be used to image the neural connectivity of thick tissue samples due to problems such as slow imaging speed, severe photobleaching of fluorescent probes, and high background noise. Recently developed DNA-PAINT solved the photobleaching problem, but its imaging speed is extremely low. We report accelerated super-resolution fluorescence microscopy named FRET-PAINT. Compared to conventional DNA-PAINT, the imaging speed of the microscopy increases up to ~30-fold. As demonstrations, we show that 25-50 second imaging time is long enough to provide super-resolution reconstruction of microtubules and mitochondria of COS-7 cells.