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Accelerated super-resolution imaging with FRET-PAINT

Cited 15 time in Web of Science Cited 16 time in Scopus
Authors
Lee, Jongjin; Park, Sangjun; Kang, Wooyoung; Hohng, Sungchul
Issue Date
2017-12-28
Publisher
BioMed Central
Citation
Molecular Brain, 10(1):63
Keywords
FRET-PAINTDNA-PAINTsuper-resolution microscopySMLMsingle-molecule localization microscopy,FRETfluorescence resonance energy transfer
Abstract
Super-resolution fluorescence microscopy in the current form is hard to be used to image the neural connectivity of thick tissue samples due to problems such as slow imaging speed, severe photobleaching of fluorescent probes, and high background noise. Recently developed DNA-PAINT solved the photobleaching problem, but its imaging speed is extremely low. We report accelerated super-resolution fluorescence microscopy named FRET-PAINT. Compared to conventional DNA-PAINT, the imaging speed of the microscopy increases up to ~30-fold. As demonstrations, we show that 25-50 second imaging time is long enough to provide super-resolution reconstruction of microtubules and mitochondria of COS-7 cells.
ISSN
1756-6606
Language
English
URI
http://hdl.handle.net/10371/138478
DOI
https://doi.org/10.1186/s13041-017-0344-5
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College of Natural Sciences (자연과학대학)Dept. of Physics and Astronomy (물리·천문학부)Physics (물리학전공)Journal Papers (저널논문_물리학전공)
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