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Use of carbonate extraction in analyzing moderately hydrophobic transmembrane proteins in the mitochondrial inner membrane

Cited 22 time in Web of Science Cited 25 time in Scopus
Authors

Kim, Hayoung; Botelho, Salome Calado; Park, Kwangjin; Kim, Hyun Ah

Issue Date
2015-12
Publisher
Cold Spring Harbor Laboratory Press
Citation
Protein Science, Vol.24 No.12, pp.2063-2069
Abstract
Resistance to sodium carbonate extraction is regarded as a canonical way to distinguish integral membrane proteins (MPs) from other membrane-associated proteins. However, it has been observed that carbonate extraction releases some mitochondrial integral MPs. Here, by analyzing both artificially designed and native mitochondrial inner MPs containing transmembrane domains (TMDs) of different hydrophobicities, we show that carbonate treatment can release moderately hydrophobic TMDs from the mitochondrial inner membrane. These results suggest that resistance and sensitivity to carbonate extraction may be interpreted with caution when analyzing the nature of mitochondrial inner MPs.
ISSN
0961-8368
Language
English
URI
https://hdl.handle.net/10371/138652
DOI
https://doi.org/10.1002/pro.2817
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