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Immunologic characteristics of human gingival fibroblasts in response to oral bacteria
DC Field | Value | Language |
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dc.contributor.author | Jang, J. Y. | - |
dc.contributor.author | Song, I. -S. | - |
dc.contributor.author | Baek, K. J. | - |
dc.contributor.author | Choi, Y. | - |
dc.contributor.author | Ji, S. | - |
dc.creator | 최영님 | - |
dc.date.accessioned | 2018-01-24T05:59:43Z | - |
dc.date.available | 2020-04-05T05:59:43Z | - |
dc.date.created | 2018-08-22 | - |
dc.date.issued | 2017-06 | - |
dc.identifier.citation | Journal of Periodontal Research, Vol.52 No.3, pp.447-457 | - |
dc.identifier.issn | 0022-3484 | - |
dc.identifier.uri | https://hdl.handle.net/10371/138986 | - |
dc.description.abstract | Background and ObjectiveThere is ample evidence that gingival fibroblasts (GFs) participate in the immune response to oral bacteria and serve as immune-regulatory cells. The objective of this study was to investigate the innate immune response of GFs to oral bacteria. Material and MethodsHuman GFs were cocultured with relatively less-pathogenic (Leptotrichia wadei, Fusobacterium nucleatum and Campylobacter gracilis) and pathogenic red-complex bacteria. The expression of mRNA for antimicrobial peptides [AMPs; namely human beta defensins (HBDs)], chemokines with antimicrobial activity [chemokine C-X-C motif (CXCL)10, CXCL11 and chemokine C-C motif ligand 20 (CCL20)] and proinflammatory mediators [interleukin (IL)6 and IL8] and the levels of CXCL11, CCL20, IL-6 and IL-8 accumulated in supernatants were analyzed using real-time PCR and ELISA, respectively. The proteolytic activities of CXCL11, CCL20, IL-6 and IL-8 produced by six species of bacteria were also determined. ResultsThe relatively less-pathogenic bacteria strongly up-regulated the expression of antimicrobial chemokines and proinflammatory mediators, whereas the red-complex bacteria stimulated low levels, or often suppressed, expression of these factors. Regarding the regulation of AMPs, the inhibition of HBD3, HBD106 and HBD107 mRNAs by Porphyromonas gingivalis was noticeable; however, differences between the two bacterial groups were not conspicuous. Differential degradation of proteins by the six bacterial species was observed: P. gingivalis and Treponema denticola degraded proteins well, whereas the other species degraded proteins to a relatively lower degree. ConclusionThe invasion of red-complex bacteria into gingival connective tissue can suppress the immune response of GFs and can be a source of persistent infection in connective tissue. | - |
dc.language | 영어 | - |
dc.language.iso | en | en |
dc.publisher | Blackwell Publishing Inc. | - |
dc.title | Immunologic characteristics of human gingival fibroblasts in response to oral bacteria | - |
dc.type | Article | - |
dc.identifier.doi | 10.1111/jre.12410 | - |
dc.citation.journaltitle | Journal of Periodontal Research | - |
dc.identifier.wosid | 000399955500016 | - |
dc.identifier.scopusid | 2-s2.0-84983266557 | - |
dc.description.srnd | OAIID:RECH_ACHV_DSTSH_NO:T201632079 | - |
dc.description.srnd | RECH_ACHV_FG:RR00200001 | - |
dc.description.srnd | ADJUST_YN: | - |
dc.description.srnd | EMP_ID:A075701 | - |
dc.description.srnd | CITE_RATE:2.662 | - |
dc.description.srnd | FILENAME:2017-Immunologic characteristics of human gingival fibroblasts in response to oral bacteria.pdf | - |
dc.description.srnd | DEPT_NM:치의과학과 | - |
dc.description.srnd | EMAIL:youngnim@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | FILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/68704094-d53a-4988-a4ed-b5c52fb8581c/link | - |
dc.citation.endpage | 457 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 447 | - |
dc.citation.volume | 52 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Choi, Y. | - |
dc.identifier.srnd | T201632079 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ANTIMICROBIAL PEPTIDES | - |
dc.subject.keywordPlus | CHEMOKINE RECEPTORS | - |
dc.subject.keywordPlus | PERIODONTAL-DISEASE | - |
dc.subject.keywordPlus | TREPONEMA-DENTICOLA | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | HEALTH | - |
dc.subject.keywordPlus | DEFENSINS | - |
dc.subject.keywordPlus | INNATE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordAuthor | antimicrobial chemokines | - |
dc.subject.keywordAuthor | human gingival fibroblasts | - |
dc.subject.keywordAuthor | oral bacteria | - |
dc.subject.keywordAuthor | proinflammatory mediator | - |
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