Determination of S-(-)-lansoprazole in dexlansoprazole preparation by capillary zone electrophoresis

Abstract

Capillary zone electrophoresis was successfully applied to the enantiomeric purity determination of dexlansoprazole using sulfobutyl ether-beta-cyclodextrin and methyl-beta-cyclodextrin as chiral selectors. Separations were carried out in a 50 mu m, 64/56 cm fused-silica capillary. The optimized conditions included 90 mM phosphate buffer, pH 6.0, containing 30 mM sulfobutyl ether-beta-cyclodextrin, 20 mM methyl-beta-cyclodextrin as background electrolyte, an applied voltage of 25 kV and a temperature of 16 A degrees C, detection was at 280 nm. The assay was validated for the S-(-)-lansoprazole in the range of 0.2-1.0%. The limit of detection was 0.07%, the limit of quantitation was 0.20%, relative to a total concentration of 4.0 mg mL(-1). Intra-day precision varied between 1.72 and 2.07%. Relative standard deviations of inter-day precision ranged between 1.62 and 1.96% for peak area ratio. The assay was applied for the determination of the chiral purity of dexlansoprazole capsules. Recovery in capsules was ranged between 101.7 and 103.1%.