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Development of genome engineering technologies in cattle: from random to specific

Cited 13 time in Web of Science Cited 17 time in Scopus
Authors
Yum, Soo-Young; Youn, Ki-Young; Choi, Woo-Jae; Jang, Goo
Issue Date
2018-01-30
Publisher
BioMed Central
Citation
Journal of Animal Science and Biotechnology, 9(1):16
Keywords
CattleCRISPR-Cas9Genome engineering technologiesTransgenesisTransposon
Abstract
Abstract
The production of transgenic farm animals (e.g., cattle) via genome engineering for the gain or loss of gene functions is an important undertaking. In the initial stages of genome engineering, DNA micro-injection into one-cell stage embryos (zygotes) followed by embryo transfer into a recipient was performed because of the ease of the procedure. However, as this approach resulted in severe mosaicism and has a low efficiency, it is not typically employed in the cattle as priority, unlike in mice. To overcome the above issue with micro-injection in cattle, somatic cell nuclear transfer (SCNT) was introduced and successfully used to produce cloned livestock. The application of SCNT for the production of transgenic livestock represents a significant advancement, but its development speed is relatively slow because of abnormal reprogramming and low gene targeting efficiency. Recent genome editing technologies (e.g., ZFN, TALEN, and CRISPR-Cas9) have been rapidly adapted for applications in cattle and great results have been achieved in several fields such as disease models and bioreactors. In the future, genome engineering technologies will accelerate our understanding of genetic traits in bovine and will be readily adapted for bio-medical applications in cattle.
ISSN
2049-1891
Language
English
URI
https://hdl.handle.net/10371/139575
DOI
https://doi.org/10.1186/s40104-018-0232-6
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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