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Superresolution fluorescence microscopy for 3D reconstruction of thick samples
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Sangjun | - |
dc.contributor.author | Kang, Wooyoung | - |
dc.contributor.author | Kwon, Yeong-Dae | - |
dc.contributor.author | Shim, Jaehoon | - |
dc.contributor.author | Kim, Siyong | - |
dc.contributor.author | Kaang, Bong-Kiun | - |
dc.contributor.author | Hohng, Sungchul | - |
dc.date.accessioned | 2018-04-18T08:09:17Z | - |
dc.date.available | 2018-04-18T17:11:56Z | - |
dc.date.issued | 2018-03-15 | - |
dc.identifier.citation | Molecular Brain, 11(1):17 | ko_KR |
dc.identifier.issn | 1756-6606 | - |
dc.identifier.uri | https://hdl.handle.net/10371/139695 | - |
dc.description.abstract | Abstract
Three-dimensional (3D) reconstruction of thick samples using superresolution fluorescence microscopy remains challenging due to high level of background noise and fast photobleaching of fluorescence probes. We develop superresolution fluorescence microscopy that can reconstruct 3D structures of thick samples with both high localization accuracy and no photobleaching problem. The background noise is reduced by optically sectioning the sample using line-scan confocal microscopy, and the photobleaching problem is overcome by using the DNA-PAINT (Point Accumulation for Imaging in Nanoscale Topography). As demonstrations, we take 3D superresolution images of microtubules of a whole cell, and two-color 3D images of microtubules and mitochondria. We also present superresolution images of chemical synapse of a mouse brain section at different z-positions ranging from 0μm to 100μm. | ko_KR |
dc.description.sponsorship | This work was supported by a Creative Research Initiative grant (Physical Genetics Laboratory, 2009–0081562) to SH and by National Honor Scientist Program to BK (NRF2012R1A3A1050385) from National Research Foundation of Korea. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BioMed Central | ko_KR |
dc.subject | Line-scan confocal microscopy | ko_KR |
dc.subject | DNA-PAINT | ko_KR |
dc.subject | Superresolution microscopy | ko_KR |
dc.subject | Single-molecule localization | ko_KR |
dc.subject | microscopy | ko_KR |
dc.subject | Three-dimensional reconstruction | ko_KR |
dc.title | Superresolution fluorescence microscopy for 3D reconstruction of thick samples | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 박상준 | - |
dc.contributor.AlternativeAuthor | 강우영 | - |
dc.contributor.AlternativeAuthor | 권영대 | - |
dc.contributor.AlternativeAuthor | 심재훈 | - |
dc.contributor.AlternativeAuthor | 김시용 | - |
dc.contributor.AlternativeAuthor | 강봉근 | - |
dc.contributor.AlternativeAuthor | 홍성철 | - |
dc.identifier.doi | 10.1186/s13041-018-0361-z | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s). | - |
dc.date.updated | 2018-03-18T04:13:29Z | - |
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