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Studies on application of primordial germ cells for interspecies chimera and molecular characterization of NANOG in aves : 이종간 조류 키메라 생산을 위한 생식세포의 활용 및 NANOG 분석을 통한 생식세포의 특성 규명
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 한재용 | - |
| dc.contributor.author | 최희정 | - |
| dc.date.accessioned | 2018-05-28T16:34:28Z | - |
| dc.date.available | 2019-04-17 | - |
| dc.date.issued | 2018-02 | - |
| dc.identifier.other | 000000150925 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/140794 | - |
| dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 농업생명과학대학 농생명공학부, 2018. 2. 한재용. | - |
| dc.description.abstract | Avian is one of the excellent models for basic and applied
research such as reproductive system and developmental biology. In particular, avian primordial germ cells (PGCs) have unique characteristics and accessibility compared to mammals during early embryo development. Thus, the production of germline chimera has been regarded as the most powerful tool via PGCs-mediated technology. In addition, a detailed understanding of germ cells has been emphasized for maintaining distinct properties in germ cell biology. In this regards, we achieved the combination of the unique migratory route of avian germ cells with an embryo replacement method could be applied to produce interspecies germline chimeras and developed potential knowledge for the conservation of wild and endangered birds through surrogate system. Furthermore, we investigated the structural characteristics of chicken NANOG (cNANOG) protein and its biological significance using biochemical methods and cell assay in PGCs. Also, we revealed the oviduct specific genes regulated by estrogen for potential therapeutic peptides or regulation of egg white protein secretion. | - |
| dc.description.tableofcontents | CHAPTER 1. GENERAL INTRODUCTION 1
CHAPTER 2. LITERATURE REVIEW 5 1. Strategies for Producing Germline Chimera in Avian 6 1.1 Blastoderm cells mediated Germline Chimera 6 1.2 Spermatogonial stem cells mediated Germline Chimera 7 1.3 Primordial germ cells mediated Germline Chimera 8 1.3.1 Biological Characteristic of PGCs 8 1.3.2 PGCs-mediated Germline Chimera in avian 12 2. Production of Interspecies Germline Chimera 13 2.1. Quail to Chicken 13 2.2 Turkey to Chicken 14 2.3 Duck to Chicken and Chicken to Duck 14 2.4 Others 15 3. PGCs-specific Genes and their Regulation 15 3.1. Regulatory Mechanism of NANOG gene 16 3.1.1 Molecular Characteristic of NANOG 16 3.1.2 Regulation of NANOG 17 3.1.3 Function of NANOG Protein 18 3.2. Regulatory Mechanism of VASA gene 19 3.3 Regulatory Mechanism of DAZL gene 21 4. Estrogen Regulation of Gene in Chicken Oviduct 22 4.1. Molecular Mechanisms of Estrogen Action 22 4.2 Effect of Estrogen in the Chicken Oviduct 22 CHAPTER 3. PRODUCTION OF INTERSPECIFIC GERMLINE CHIMERAS VIA EMBRYO REPLACEMENT 25 1. Introduction 26 2. Materials and methods 29 3. Results 32 4. Discussion 43 5. Conclusion 46 CHAPTER 4. NORMAL DEVELOPMENT AND HATCHABILITY OF KOREAN OGE CHICKENS IN WHITE LEGHORN SURROGATE EGGSHELLS 47 1. Introduction 50 2. Materials and methods 52 3. Results 54 4. Discussion 60 5. Conclusion 62 CHAPTER 5. CHICKEN NANOG SELF-ASSOCIATES VIA A NOVEL FOLDING-UPON-BINDING MECHANISM 63 1. Introduction 64 2. Materials and methods 66 3. Results 72 4. Discussion 89 5. Conclusion 93 CHAPTER 6. DISCOVERY OF NOVEL GENE EXPRESSED IN MAGNUM TISSUE OF CHICKEN OVIDUCT 95 1. Introduction 96 2. Materials and methods 98 3. Results 102 4. Discussion 110 5. Conclusion 112 CHAPTER 7. GENERAL DISCUSSION 113 REFERENCES 117 SUMMARY IN KOREAN 149 | - |
| dc.format | application/pdf | - |
| dc.format.extent | 7935139 bytes | - |
| dc.format.medium | application/pdf | - |
| dc.language.iso | en | - |
| dc.publisher | 서울대학교 대학원 | - |
| dc.subject | chicken | - |
| dc.subject | interspecies germline chimera | - |
| dc.subject | primordial germ cells | - |
| dc.subject | NANOG | - |
| dc.subject | oviduct | - |
| dc.subject.ddc | 630 | - |
| dc.title | Studies on application of primordial germ cells for interspecies chimera and molecular characterization of NANOG in aves | - |
| dc.title.alternative | 이종간 조류 키메라 생산을 위한 생식세포의 활용 및 NANOG 분석을 통한 생식세포의 특성 규명 | - |
| dc.type | Thesis | - |
| dc.description.degree | Doctor | - |
| dc.contributor.affiliation | 농업생명과학대학 농생명공학부 | - |
| dc.date.awarded | 2018-02 | - |
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