S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) Theses (Ph.D. / Sc.D._의학과)
Effects of short-term exposure to sevoflurane on the survival, proliferation, apoptosis, and differentiation of neural precursor cells derived from human embryonic stem cells
사람 배아 줄기세포로부터 얻어낸 신경 전구세포의 생존, 증식, 아포토시스, 분화에 대한 세보플루란 단기 노출의 영향
- 의과대학 의학과
- Issue Date
- 서울대학교 대학원
- 학위논문 (박사)-- 서울대학교 대학원 : 의과대학 의학과, 2018. 2. 김진희.
- Purpose: Data from animal experiments suggest that exposure to general anesthetics in early life inhibits neurogenesis and causes long-term memory deficit. Regarding a short operating time and popularity of sevoflurane in pediatric anesthesia, it is important to verify effects of short period exposure to sevoflurane on developing brain.
Methods: We measured the effects of short-term exposure (2 h) to 3%, 6% or 8% sevoflurane, the most commonly used anesthetic, on neural precursor cells derived from human embryonic stem cells, SNUhES32. On days 1, 3, 5 and 7 post-treatment, cell survival, proliferation, apoptosis and differentiation were analyzed.
Results: Treatment with 6% sevoflurane increased cell viability (P = 0.046) and decreased apoptosis (P = 0.014) on day 5, but didnt last on day 7. Survival and apoptosis were not affected by 3% and 8% sevoflurane
there was no effect of proliferation at any of the tested concentrations. The differentiation of cells exposed to 6% or 8% sevoflurane decreased on day 1 (P = 0.033 and 0.036 for 6% and 8% sevoflurane, respectively) but was again normalized on days 3–7.
Conclusion: The clinically relevant treatment with sevoflurane for 2 h induces no significant changes of the survival, proliferation, apoptosis and differentiation of human neural precursor cells, although supra-clinical doses of sevoflurane alter human neurogenesis transiently.