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Helicobacter pylori is associated with miR-133a expression through promoter methylation in gastric carcinogenesis : 헬리코박터 파일로리가 위암 발병에서 프로모터 메틸화를 통해 miR-133a 발현에 미치는 영향
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김상균 | - |
dc.contributor.author | 임주현 | - |
dc.date.accessioned | 2018-05-28T16:59:27Z | - |
dc.date.available | 2018-05-28T16:59:27Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.other | 000000149311 | - |
dc.identifier.uri | https://hdl.handle.net/10371/141023 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 의과대학 의학과, 2018. 2. 김상균. | - |
dc.description.abstract | Background/Aims: To investigate whether Helicobacter pylori eradication can reverse epigenetic silencing of miRNAs which are associated with H. pylori-induced gastric carcinogenesis.
Methods: We examined expression and promoter methylation of miR-34b/c, miR-133a, let-7a, and let-7i in gastric cancer cell line, before/after demethylation. Among them, epigenetically controlled miRNAs were identified. Their expression and promoter methylation was examined in human tissues of H. pylori-positive gastric cancer(T), H. pylori-positive gastritis(H), and H. pylori-negative controls(C). We also compared changes of miRNA expression and promoter methylation in H. pylori-positive patients who were endoscopically treated for early gastric cancer, between baseline and 1 year later according to eradication status. Results: In gastric cancer cell line, miR-34b/c, and miR-133a showed epigenetic silencing. In human tissues, miR-34b/c and miR-133a showed serial increase of promoter methylation in order of C, H, and T (all, p<0.01), and the miR-133a expression showed serial decrease (C vs. H, p=.02 | - |
dc.description.abstract | H vs. T, 0.01 | - |
dc.description.abstract | C vs T | - |
dc.description.abstract | p<0.01) while miR-34b and miR-34c expressions did not. H. pylori eradication induced decrease of methylation (p<0.01) and increase of miR-133a expression (p=0.03), compared with non-eradication group.
Conclusions: This result suggests H. pylori eradication could reverse methylation-silencing of miR-133a which is involved in H. pylori-induced gastric carcinogenesis. | - |
dc.description.tableofcontents | Introduction 1
Materials and Methods 4 Results 11 Discussion 30 References 36 Abstract in Korean 44 | - |
dc.format | application/pdf | - |
dc.format.extent | 538104 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Helicobacter pylori | - |
dc.subject | methylation | - |
dc.subject | microRNA | - |
dc.subject | gastric cancer | - |
dc.subject.ddc | 610 | - |
dc.title | Helicobacter pylori is associated with miR-133a expression through promoter methylation in gastric carcinogenesis | - |
dc.title.alternative | 헬리코박터 파일로리가 위암 발병에서 프로모터 메틸화를 통해 miR-133a 발현에 미치는 영향 | - |
dc.type | Thesis | - |
dc.description.degree | Doctor | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2018-02 | - |
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