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Periostin Induces Kidney Fibrosis after Acute Kidney Injury via p38 MAPK pathway
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 임춘수 | - |
dc.contributor.author | 안정남 | - |
dc.date.accessioned | 2018-05-28T17:02:26Z | - |
dc.date.available | 2018-05-28T17:02:26Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.other | 000000151466 | - |
dc.identifier.uri | https://hdl.handle.net/10371/141057 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 의과대학 임상의과학과, 2018. 2. 임춘수. | - |
dc.description.abstract | Background: Periostin, a matricellular protein, has been reported to play a crucial role in fibrosis. Acute kidney injury results in a high risk of progression to chronic kidney disease. It was hypothesized that periostin is involved in the progression of acute kidney injury to kidney fibrosis.
Methods: Unilateral ischemia-reperfusion injury using 7- to 8-week-old male wild-type and periostin null mice was induced and the animals were observed for 6 weeks. In vitro, human kidney-2 cells were subjected to a hypoxic incubator (5% O2, 5% CO2, and 90% N2) for 24 hours and 5 days. The cells were also cultured with a p38 mitogen-activated protein kinase (MAPK) inhibitor in the hypoxic incubator for 5 days. Results: At 6 weeks after induction of unilateral ischemia-reperfusion injury, the kidneys in periostin null mice were less atrophied, and interstitial fibrosis/tubular atrophy was significantly alleviated in periostin null mice compared with those in wild-type mice. The expressions of phosphorylated-p38 MAPK were also decreased in periostin null mice compared to those in wild-type mice. Furthermore, periostin null mice had attenuated mRNA and protein expression of fibrosis and apoptosis markers. In vitro, hypoxic injury (5% O2, 5% CO2, and 90% N2) of the human kidney-2 cells for 24 hours and 5 days increased the expressions of phosphorylated-p38 MAPK and fibrosis markers. Recombinant periostin in hypoxic conditions enhanced phosphorylated-p38 MAPK expression, which was comparable to that with recombinant transforming growth factor-β1. In contrast, inhibition of p38 MAPK ameliorated hypoxia-induced fibrosis. Conclusion: In conclusion, periostin promotes kidney fibrosis via the p38 MAPK pathway following acute kidney injury triggered by hypoxic or ischemic insult. Periostin ablation could protect against kidney progression. | - |
dc.description.tableofcontents | Introduction 1
Materials and Methods 3 Results 8 Discussion 25 Reference 29 Abstract (Korean) 34 | - |
dc.format | application/pdf | - |
dc.format.extent | 1390266 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | periostin | - |
dc.subject | acute kidney injury | - |
dc.subject | kidney fibrosis | - |
dc.subject | hypoxia | - |
dc.subject | periostin null mice | - |
dc.subject | unilateral ischemia-reperfusion injury | - |
dc.subject | p38 mitogen-activated protein kinase | - |
dc.subject.ddc | 610 | - |
dc.title | Periostin Induces Kidney Fibrosis after Acute Kidney Injury via p38 MAPK pathway | - |
dc.type | Thesis | - |
dc.description.degree | Doctor | - |
dc.contributor.affiliation | 의과대학 임상의과학과 | - |
dc.date.awarded | 2018-02 | - |
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