Comparison of the osteogenic potential and fibroblast response of 3 mol% yttria-stabilized tetragonal zirconia polycrystals and niobium oxide containing zirconia discs
Niobium oxide를 포함한 지르코니아와 yttria-stabilized 지르코니아의 골형성능과 연조직 반응에 대한 비교 연구
- Jung-Suk Han
- 치의학대학원 치의과학과
- Issue Date
- 서울대학교 대학원
- 학위논문 (박사)-- 서울대학교 대학원 : 치의학대학원 치의과학과, 2018. 2. Jung-Suk Han.
- Objective: This in vitro study evaluated the osteogenic potential and human gingival fibroblast response of 3 mol% yttria-stabilized tetragonal zirconia polycrystals (3Y-TZP) and niobium oxide containing Y-TZPs ((Y, Nb)-TZPs) with specific ratios, namely YN4533 and YN4533/Al20 discs.
Materials and Methods: The surface properties of smooth 3Y-TZP, YN4533, YN4533/Al20 and rough discs (15 mm in diameter, 1 mm thick, 20 discs for each group) were evaluated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Murine pre-osteoblast MC3T3-E1 cells and human gingival fibroblast cells were cultured on all zirconia discs. Cell attachment was evaluated by CLSM and SEM after twenty four hours of cell culturing. The PicoGreen assay was used to investigate cell proliferation at 1, 4 and 7 days for osteoblasts and 4, 24, 48 and 72 hours for fibroblasts. The mRNA gene expression of alkaline phosphatase (Alp) and osteocalcin was measured by real-time RT-PCR at 5, 8 and 11 days. Alp activity was also evaluated at the protein level. Moreover, the mRNA gene expressions of type I collagen, integrin α2 and β1 were measured by real-time RT-PCR at 4, 24 and 48 hours. Two-way ANOVA was used for the comparison of the outcomes among the groups. All the data were analyzed at the significance level of 0.05.
Results: Both MC3T3-E1 pre-osteoblasts and human gingival fibroblasts were more widely spread on smooth surfaces than on rough surfaces. Cellular proliferations of both osteoblasts and fibroblasts were higher on smooth surfaces. Fibroblasts were more proliferated on smooth YN4533 and YN4533/Al20 than smooth 3Y-TZP while no significant differences were found in osteoblasts. Osteoblasts were slightly more differentiated on YN4533 and YN4533/Al20 than on 3Y-TZP, considering the results of RT-PCR and Alp activity. The mRNA expressions of type I collagen, integrin α2 and β1 were significantly stimulated for (Y, Nb)-TZP groups at 24 hours after seeding.
Conclusions: Within the limitation of this study, (Y, Nb)-TZPs provide appropriate surface condition for osseointegration at the implant level and for peri-implant mucosal sealing at the abutment level. The (Y, Nb)-TZP is anticipated to be a suitable candidate material for ceramic dental implant with a favorable clinical outcome.