Bioglas-incorporated gelatin based cryogel for osteogenic commitment of mesenchymal stem cell

Abstract

Bioglass is widely used in medical field as an excellent biomaterial due to its osteo-inducing effect and biocompatibility. However, due to its brittleness, bioglass alone is not an ideal material for implantation. Characteristics of ideal scaffold using in bone tissue engineering are biocompatibility, high pore size, biodegradability, and mechanical property. Methacrylated gelatin (GelMA), which is a modified gelatin form, retains cell adhesion site, which is a well-suited material for cryogel fabrication. Herein, we fabricated bioglass-incorporated GelMA cryogel for testing osteogenic effect. We demonstrated that as doses of bioglass concentration in scaffolds increases, osteogenic effect on mesenchymal cells increases in in vitro testing. Furthermore, in vivo testing supports that bioglass incorporated GelMA cryogel induces stronger osteogenic differentiation on mesenchymal stem cells than the control group, GelMA cryogel. In this paper, the enhancement of osteogenic effect using bioglass embedded GelMA scaffold will be presented.