Publications
Detailed Information
Production of L-carnitine Enhanced Fermented Quinoa with Rhizopus oligosporus and its Bioactive Properties
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김도만 | - |
dc.contributor.author | 허재원 | - |
dc.date.accessioned | 2018-05-29T03:45:20Z | - |
dc.date.available | 2019-04-17 | - |
dc.date.issued | 2018-02 | - |
dc.identifier.other | 000000149665 | - |
dc.identifier.uri | https://hdl.handle.net/10371/141682 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 국제농업기술대학원 국제농업기술학과, 2018. 2. 김도만. | - |
dc.description.abstract | Quinoa is a pseudocereal that contains high quality protein, minerals, vitamins, polyphenols, and phytosterols. In this study, quinoa was fermented by Rhizopus oligosporus (R.oligosporus) up to 5 days and degradation of quinoa starch granules were observed with a scanning electron microscope. After optimization of fermentation based on L-carnitine production, L-carnitine and GABA amounts were 3.14 mg/kg and 1.04 g/kg at the third day. Total phenolic contents and total flavonoids contents were 8.0 mg gallic acid (GAE)/100 g and 1.9 mg quercetin equivalent (QE) /100 g for five days fermented quinoa (5F), and 4.1 mg GAE/100 g and 1.3 mg QE/100 g for regular quinoa (NF), respectively. Antioxidant activity (SC50) was 2.3 mg/mL for 5F and 3.6 mg/mL for NF. Nitric oxide production on RAW264.7 macrophages of fermented quinoa revealed 29.3% inhibition of nitric oxide production of NF and 56.4% for 5F, resulting in improvement 1.9 times in anti-inflammatory activity.
Fatty acids composition in fermented quinoa was increased | - |
dc.description.abstract | myristic acid (68%), palmitic acid (48%), palmitoleic acid (264%), margaric acid (25%), stearic acid (129%), linoleic acid (33%) and linolenic acid (32%), compared to the regular quinoa. As for lipid soluble extract, wound healing effects on scratched HaCaT keratinocyte were enhanced from 24.8% wound closure effects of NL to 67.5% and 58.3% of 3L and 5L, respectively. | - |
dc.description.tableofcontents | Introduction 1
1. Quinoa 1 2. L-carnitine 1 3. γ - aminobutyric acid (GABA) 2 4. Rhizopus oligosporus fermentation 2 5. Antioxidant activity of phenolic compounds 3 6. Research perspective of functional quinoa 3 Materials and Methods 5 1. Microbial strain and culture condition 5 2. Preparation of fermented quinoa 5 3. Sample extraction 6 4. Scanning electron microscopy image analyses 6 5. Analyses of L-carnitine and GABA 7 6. Analyses of phenolic acids 8 7. Total phenolic contents analyses (TPC) 9 8. Total flavonoid contents (TFC) analyses 9 9. Determination of DPPH radical scavenging activity 10 10. Cell cytotoxicity tests 11 11. Measurement of nitric oxide production 12 12. GC-FID analyses 13 13. Scratch wound healing assay 13 14. Statistical analyses 14 Results 15 1. Scanning electron microscopic observation 15 2. Ethanol extraction of fermented quinoa 18 3. Analyses of phenolic acids, L-carnitine and GABA 20 4. Total phenol content, total flavonoid contents, and antioxidant activity of quinoa 24 5. Cell viability of RAW264.7 cells 26 6. Nitric oxide production 26 7. GC-FID analyses 28 8. Wound healing effect on HaCaT cells 30 Discussion 32 Conclusion 37 References 38 Abstract in Korean 41 | - |
dc.format | application/pdf | - |
dc.format.extent | 1499900 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Anti-inflammatory activity | - |
dc.subject | Antioxidant activity | - |
dc.subject | Fermentation | - |
dc.subject | L-carnitine | - |
dc.subject | Rhizopus oligosporus | - |
dc.subject | Quinoa | - |
dc.subject.ddc | 631 | - |
dc.title | Production of L-carnitine Enhanced Fermented Quinoa with Rhizopus oligosporus and its Bioactive Properties | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 국제농업기술대학원 국제농업기술학과 | - |
dc.date.awarded | 2018-02 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.