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Identification and Inheritance of New Sources of Resistance against Cucumber mosaic virus Isolate P1 in Capsicum and Development of a Tomato yellow leaf curl Kanchanaburi virus Infectious Clone : 고추에서의 오이모자이크병 P1분리균주에 대한 새로운 저항성 자원의 발견과 유전양상 및 토마토황화잎말림 Kanchanaburi 바이러스의 감염 클론 개발
DC Field | Value | Language |
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dc.contributor.advisor | 강병철 | - |
dc.contributor.author | 최슬아 | - |
dc.date.accessioned | 2018-05-29T03:59:32Z | - |
dc.date.available | 2020-02-03T23:55:57Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.other | 000000150452 | - |
dc.identifier.uri | https://hdl.handle.net/10371/141819 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 농업생명과학대학 식물생산과학부, 2018. 2. 강병철. | - |
dc.description.abstract | Peppers (Capsicum spp.) are important vegetable and spice crop worldwide. It possesses rich accumulations of secondary metabolites, colors and certain essential vitamins and minerals. Viral diseases are severe threat to both hot and sweet peppers resulting in significant yield loss. In the present study, two pepper viruses causing such excessive yield and quality loss of pepper were studied. In the first chapter, Capsicum germplasm collection was screened to identify resistance sources of CMV-P1, a new variant of Cucumber mosaic virus (CMV) strain. No accumulation of the virus in resistant pepper accessions was verified by enzyme linked immunosorbent assay (ELISA). Among the 4,197 accessions, seven accessions of C. annuum have been screened out as new resistant sources. Pepper accessions exhibiting strong resistance against CMV-P1 were used to develop F1 population and each of them was crossed with a susceptible line C. annuum Jeju and a resistance line C. annuum Lam32, containing cmr2. The developed populations were further utilized to identify the inheritance of genetic resistance. The results suggest that the identified seven resistant accessions showed an identical pattern of genetic inheritance as the resistance gene, cmr2. The resistant resources identified in this study are expected to be applied in pepper breeding to develop CMV-P1 resistant varieties. In the second chapter, the genome of Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), a bipartite Begomovirus first reported to infect tomato and eggplant in Kanchanaburi (Thailand), was sequenced and confirmed. A TYLCKaV infectious clone was developed using the golden gate cloning method and was agroinfiltrated in tobacco, tomato and pepper plants. The virus inoculated tobacco and tomato plants displayed viral symptoms. However, no obvious TYLCKaV symptoms were observed in pepper. Accumulation of the virus was also confirmed using polymerase chain reaction (PCR). The developed TYLCKaV infectious clone will be a useful tool for studies on TYLCKaV resistance. | - |
dc.description.tableofcontents | GENERAL INTRODUCTION.1
LITERATURE REVIEW 4 1. Plant resistance to pathogen 4 2. Genetics of plant virus resistance 5 3. Cucumber mosaic virus 7 4. Tomato yellow leaf curllike virus 7 CHAPTER ⅠIdentification and Inheritance of New Sources of Resistance against Cucumber mosaic virus Isolate P1 in Capsicum 15 ABSTRACT 15 INTRODUCTION 17 MATERIALS AND METHODS 19 Plant materials 19 Virus inoculation 19 Detection of CMV accumulation using DAS-ELISA 20 Testing Affy4 marker 20 RESULTS 22 Germplasm screening 22 Inheritance study and allelism test of the new source of resistance against the CMV-P1 isolate 26 cmr2-linked Affy4 marker analysis 30 DISCUSSION 32 REFERENCES 36 CHAPTER Ⅱ Development of a Tomato yellow leaf curl Kanchanaburi virus Infectious Clone 39 ABSTRACT 39 INTRODUCTION 41 MATERIALS AND METHODS 43 Plant materials 43 Virus inoculation 43 Detection and sequencing of the viral genomes 43 Construction of an infectious clone 44 Validation of infectious TYLCKaV clone 47 DNA extraction and electrophoresis 47 RESULTS 50 Detection and sequence analysis of the TYLCKaV 50 Infectivity test of TYLCKaV infectious clone in tobacco 55 Infectivity test of TYLCKaV infectious clone in tomato 57 Infectivity test of TYLCKaV infectious clone in pepper 61 DISCUSSION 65 REFERENCES 69 ABSTRACT IN KOREAN 74 | - |
dc.format | application/pdf | - |
dc.format.extent | 1607657 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Cucumber mosaic virus (CMV) | - |
dc.subject | Tomato yellow leaf curl Kanchanaburk virus (TYLCKaV) | - |
dc.subject | infectious clone | - |
dc.subject | Capsicum | - |
dc.subject | Pepper virus | - |
dc.subject.ddc | 633 | - |
dc.title | Identification and Inheritance of New Sources of Resistance against Cucumber mosaic virus Isolate P1 in Capsicum and Development of a Tomato yellow leaf curl Kanchanaburi virus Infectious Clone | - |
dc.title.alternative | 고추에서의 오이모자이크병 P1분리균주에 대한 새로운 저항성 자원의 발견과 유전양상 및 토마토황화잎말림 Kanchanaburi 바이러스의 감염 클론 개발 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Seula Choi | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 농업생명과학대학 식물생산과학부 | - |
dc.date.awarded | 2018-02 | - |
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