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Inhibitory effect of Lactobacillus plantarum isolated from intestinal microbiota on Clostridium difficile infection
DC Field | Value | Language |
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dc.contributor.advisor | 고광표 | - |
dc.contributor.author | 유준선 | - |
dc.date.accessioned | 2018-05-29T04:14:07Z | - |
dc.date.available | 2021-04-13T01:54:37Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.other | 000000151187 | - |
dc.identifier.uri | https://hdl.handle.net/10371/141938 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 보건대학원 환경보건학과, 2018. 2. 고광표. | - |
dc.description.abstract | Clostridium difficile infection (CDI) is one of the most widely known nosocomial infections. The dysbiosis of gut microbiota due to antibiotic use is the leading cause of CDI | - |
dc.description.abstract | people with relatively healthy gut microbiota do not typically become infected with CDI. It is, therefore, necessary for researchers to find a specific bacterium that inhibits CDI among healthy gut microbiota to regulate it. However, there have been few studies about CDI-resistant strains among healthy gut microbiota. Therefore, this study aims to verify the inhibitory effect of single strains isolated from normal individuals on CDI and to determine the underlying mechanism. Through previous studies, three strains isolated from healthy Koreans with C. difficile inhibitory effects were selected. The CDI inhibitory effects of the three strains were confirmed through animal experimentation. CDI inhibition was assessed by post-infection weight change, C. difficile colony forming unit (c.f.u.), and the toxin A&B measured value. The feces collected in the animal experiment were used to analyze the changes and status of gut microbiota and gut metabolome. As a result of this experiment, a significant inhibition of CDI was observed in the group to which Lactobacillus plantarum SNUG 10271 was administered. Gut microbiota analysis showed that Ruminococcaceae and Coprococcus showed high abundance in the L. plantarum SNUG 10271 group and was negatively correlated with C. difficile. Gut metabolome analysis showed that the concentration of deoxycholic acid (DCA) was significantly higher in individuals with inhibitory effects. These results confirmed the possibility of CDI inhibition of a single strain isolated from healthy individuals. We also found information on Ruminococcaceae, Coprococcus, and DCA that will be necessary to understand the mechanism of CDI inhibition. | - |
dc.description.tableofcontents | I.Introduction 9
II.Materials and Methods 13 1.Culture condition of bacteria 13 2.Sporulation of C. difficile 13 3.Murine C. difficile infection time-course experiment 14 4.Measurement of C. difficile c.f.u. and toxin A&B 16 5.Fecal microbiota analysis 17 6.Quantification of DCA and metabolome analysis of fecal samples 18 7.Statistical analysis 19 III.Results 21 1.L. plantarum SNUG 10271 inhibit clinical symptoms of CDI 21 2.Comparison of gut microbial diversity 26 3.The characterization of specific bacterial taxa in the group with the CDI inhibitory effect 29 4.CDI-related functional profile of gut microbiota 33 5.The characterization of specific bacterial metabolites in the group with a CDI inhibitory effect 35 6.The correlation of gut microbiota with bile acids 39 IV.Discussion 43 V.Reference 50 VI.국문초록 55 | - |
dc.format | application/pdf | - |
dc.format.extent | 3068634 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Clostridium difficile infection | - |
dc.subject | Microbiota | - |
dc.subject | Lactobacillus plantarum SNUG 10271 | - |
dc.subject | Ruminococcaceae | - |
dc.subject | Coprococcus | - |
dc.subject | Deoxycholic acid | - |
dc.subject.ddc | 363.7 | - |
dc.title | Inhibitory effect of Lactobacillus plantarum isolated from intestinal microbiota on Clostridium difficile infection | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 보건대학원 환경보건학과 | - |
dc.date.awarded | 2018-02 | - |
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