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Reactive Oxygen Species (ROS) activity of ambient fine particles (PM2.5) measured in Seoul, Korea
서울의 대기 중 초미세먼지의 Reactive Oxygen Species (ROS) activity 분석

DC Field Value Language
dc.contributor.advisor이승묵-
dc.contributor.author박지은-
dc.date.accessioned2018-05-29T04:14:41Z-
dc.date.available2018-05-29T04:14:41Z-
dc.date.issued2018-02-
dc.identifier.other000000151436-
dc.identifier.urihttps://hdl.handle.net/10371/141944-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 보건대학원 환경보건학과, 2018. 2. 이승묵.-
dc.description.abstract대기 중 미세먼지의 증가는 호흡기계 및 심혈관계 질환에 의한 사망률과 유병률에 큰 영향을 미치며, World Health Organization (WHO)의 International Agency for Research on Cancer (IARC)는 미세먼지를 폐암을 유발하는 1급 발암물질로 보고하였다. 그 중에서도 직경이 2.5㎛ 이하인 초미세먼지(PM2.5)는 작은 크기 때문에 폐포 깊숙이 침투하여 흡수되기 용이한 것으로 알려져 있고, 이 때문에 초미세먼지가 미세먼지보다 건강영향에 더 많은 악영향을 준다고 보고되고 있다. 현재까지 우리나라에서는 초미세먼지와 건강영향에 대한 역학적 연구는 수행되어왔지만 질병들의 시초가 되는 세포단위의 노출평가는 아직 진행된 바가 없다. 따라서 본 연구는 서울에서 채취한 대기 중 초미세먼지의 화학성분 및 오염원을 도출하고 Reactive Oxygen Species (ROS) activity와의 상관성을 분석하는데 초점을 맞추었다. PM2.5의 농도는 계절을 cold season과 warm season으로 나누었을 때 cold season 일 때 높게 나타났으며, Secondary Organic Carbon (SOC)과 SO42-를 제외한 나머지 화학 종들도 같은 양상을 보였다. 그 중에서도 cold season 일 때 금속성분 중 지각성분이 전체 PM2.5 농도의 20%를 차지하는 것으로 보아 국외에서 유입된 황사 스모그의 영향을 받았을 가능성이 높은 것으로 사료된다. 각각의 화학 종들과 ROS activity와의 상관계수분석을 통해 Water Soluble Organic Carbon (WSOC)과 수용성 전이금속인 Cr과 Zn가 높은 상관성을 보이는 것을 확인하였고, 단계적 다중 선형 회귀분석을 통하여 As과 V이 높은 상관성을 보이는 것을 확인하였다. 주성분분석을 통하여 soil, mobile, industry, secondary inorganic aerosol (SIA), secondary organic aerosol (SOA), 그리고 oil combustion 등 6개의 오염원을 도출하였고, 그 중에서도 industry, soil, mobile 그리고 SIA과 ROS activity가 유의한 상관성을 보였다.-
dc.description.abstractSubstantial increase in level of particulate matter has raised concerns in South Korea recently. Ambient particulate matter is classified as Group I carcinogen (IARC, 2013) and multiple epidemiological studies has demonstrated adverse health effects due to exposure of particulate matter. Fine particulate matter (PM2.5) which has a diameter less than 2.5 µm is likely to penetrate deeply into lung and is known to be eliciting adverse health effects. A number of epidemiological studies have been conducted on adverse health effects of PM-related diseases and mortality rate, yet particulate matter (PM)-induced reactive oxygen species (ROS) activity at the cellular level has not been actively studied in Korea. This study assessed PM-induced oxidative potential by exposure of collected ambient PM2.5 samples to the rat alveolar macrophage cell line. The characteristics of PM2.5 in Korea were further characterized by linking chemical constituents and contributing sources to ROS. PM2.5 mass concentration during the cold season was relatively higher than mass concentration during the warm season and chemical constituents except for Secondary Organic Carbon (SOC) and SO42- followed similar trends. The concentration of crustal elements was especially high during the cold season which can be an indication of long range transport of Asian dust. Water soluble organic carbon and water soluble transition metals (Cr and Zn) were also shown to be correlated to oxidative potential and metals such as As and V were shown to have a high contribution to ROS activity according to stepwise multiple linear regression. Principal Component Analysis (PCA) results identified six factors that can be interpreted as soil, mobile, industry, secondary inorganic aerosol, secondary organic aerosol and oil combustion. Moreover, through Principal Component Regression (PCR), industry, soil, mobile and SIA were shown to be statistically significant sources in a relation to ROS activity.-
dc.description.tableofcontentsI.Introduction 1
II.Experimental Methods 4
1.Sample collection and analysis 4
2.Macrophage ROS analysis 6
3.Data analysis 7
III.Results 8
1.Chemical constituents 8
2.Correlation between ROS activity and chemical species 14
3.Stepwise Multiple Linear Regression 16
4.Association of ROS activity and sources 18
4.1.Principal Component Analysis 18
4.2.Principal Component Regression 20
IV.Discussion and Conclusion 21
V.References 28
VI.국문초록 35
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dc.formatapplication/pdf-
dc.format.extent1145538 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectFine particulate matter (PM2.5)-
dc.subjectChemical constituents-
dc.subjectReactive Oxygen Species (ROS)-
dc.subjectHealth effect-
dc.subject.ddc363.7-
dc.titleReactive Oxygen Species (ROS) activity of ambient fine particles (PM2.5) measured in Seoul, Korea-
dc.title.alternative서울의 대기 중 초미세먼지의 Reactive Oxygen Species (ROS) activity 분석-
dc.typeThesis-
dc.contributor.AlternativeAuthorJieun Park-
dc.description.degreeMaster-
dc.contributor.affiliation보건대학원 환경보건학과-
dc.date.awarded2018-02-
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Graduate School of Public Health (보건대학원)Dept. of Environmental Health (환경보건학과)Theses (Master's Degree_환경보건학과)
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