Fibroblast growth factor-1 as a mediator of paracrine effects of canine adipose tissue-derived mesenchymal stem cells on in vitro-induced insulin resistance models : In vitro 인슐린 저항성 모델에 대한 개 지방유래중간엽줄기세포 paracrine 효과의 매개체 fibroblast growth factor-1

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수의과대학 수의학과
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fibroblast growth factor-1canine adipose tissue-derived mesenchymal stem cellsconditioned mediumdiabetes mellitusinsulin resistancein vitro3T3-L1
학위논문 (석사)-- 서울대학교 대학원 : 수의과대학 수의학과, 2018. 2. 윤화영.
In the field of diabetes research, many studies on cell therapy have been conducted using mesenchymal stem cells. This research was intended to shed light on the influence of canine adipose-tissue-derived mesenchymal stem cell conditioned medium (cAT-MSC CM) on in vitro insulin resistance models that were induced in differentiated 3T3-L1 adipocytes and the possible mechanisms involved in the phenomenon. Gene expression levels of insulin receptor substrate-1 (IRS-1) and glucose transporter type 4 (GLUT4) were used as indicators of insulin resistance. Relative protein expression levels of IRS-1 and GLUT4 were augmented in the cAT-MSC CM treatment group compared to insulin resistance model controls, indicating beneficial effects of cAT-MSC to DM, probably by actions of secreting factors. With reference to previous studies on fibroblast growth factor-1 (FGF1), it was proposed as a contributing factor to the mechanism of action. Anti-FGF1 neutralizing antibody was added to the CM-treated insulin resistance models. As a result, significantly diminished protein levels of IRS-1 and GLUT4 were observed, supporting our assumption. Accordingly, this study advocated the potential of MSC CM as an alternative insulin sensitizer and discovered a signalling factor associated with the paracrine effects of cAT-MSC.
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Theses (Master's Degree_수의학과)
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