S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Theses (Master's Degree_수의학과)
Fibroblast growth factor-1 as a mediator of paracrine effects of canine adipose tissue-derived mesenchymal stem cells on in vitro-induced insulin resistance models : In vitro 인슐린 저항성 모델에 대한 개 지방유래중간엽줄기세포 paracrine 효과의 매개체 fibroblast growth factor-1
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- fibroblast growth factor-1 ; canine adipose tissue-derived mesenchymal stem cells ; conditioned medium ; diabetes mellitus ; insulin resistance ; in vitro ; 3T3-L1
- 학위논문 (석사)-- 서울대학교 대학원 : 수의과대학 수의학과, 2018. 2. 윤화영.
- In the field of diabetes research, many studies on cell therapy have been conducted using mesenchymal stem cells. This research was intended to shed light on the influence of canine adipose-tissue-derived mesenchymal stem cell conditioned medium (cAT-MSC CM) on in vitro insulin resistance models that were induced in differentiated 3T3-L1 adipocytes and the possible mechanisms involved in the phenomenon. Gene expression levels of insulin receptor substrate-1 (IRS-1) and glucose transporter type 4 (GLUT4) were used as indicators of insulin resistance. Relative protein expression levels of IRS-1 and GLUT4 were augmented in the cAT-MSC CM treatment group compared to insulin resistance model controls, indicating beneficial effects of cAT-MSC to DM, probably by actions of secreting factors. With reference to previous studies on fibroblast growth factor-1 (FGF1), it was proposed as a contributing factor to the mechanism of action. Anti-FGF1 neutralizing antibody was added to the CM-treated insulin resistance models. As a result, significantly diminished protein levels of IRS-1 and GLUT4 were observed, supporting our assumption. Accordingly, this study advocated the potential of MSC CM as an alternative insulin sensitizer and discovered a signalling factor associated with the paracrine effects of cAT-MSC.
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