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Peptide-coated graphene oxide nanosheets for prolonged blood circulation : 펩타이드 수식 그래핀을 이용한 혈중 지속시간 증강연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 오유경 | - |
dc.contributor.author | 박규태 | - |
dc.date.accessioned | 2018-05-29T04:43:25Z | - |
dc.date.available | 2018-05-29T04:43:25Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.other | 000000149469 | - |
dc.identifier.uri | https://hdl.handle.net/10371/142231 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 약학대학 약학과, 2018. 2. 오유경. | - |
dc.description.abstract | In this study, we modified the surfaces of graphene oxide (GO) nanosheets with a CD47-like signal regulatory protein alpha (SIRPα)-binding peptide (SP). SIRPα is highly expressed in macrophages of the reticuloendothelial system and in tumor-associated macrophages, whereas tumor cells express the surface membrane protein, CD47, which interacts with SIRPα to negatively regulate phagocytosis. The presence of SP on GO nanosheets reduced the macrophage uptake to a greater extent than the PEGylation of such nanosheets. This reduced uptake was found to be mediated by the activation of Src homology region 2 domain-containing phosphatase 1 (SHP-1) and the downstream inhibition of myosin assembly, which is necessary for phagosome formation. Unlike SP-coated GO nanosheets, PEGylated GO nanosheets did not affect myosin assembly or phagocytosis. After in vivo systemic administration, the clearance of SP-coated GO nanosheets was slower than that of PEGylated GO nanosheets, and this difference increased with repeated administration. Finally, SP-coated GO nanosheets showed a higher distribution to tumor tissues than PEGylated GO nanosheets or a physical mixture of SP and GO nanosheets. Our findings indicate that immune-camouflaged GO nanosheets with natural CD47-like SIRPα-binding molecules can reduce the nonspecific loss of such nanosheets through macrophage uptake, thereby enhancing their blood circulation and tumor delivery after multiple injections. | - |
dc.description.tableofcontents | 1. Introduction 1
2. Materials and methods 3 3. Results 10 4. Discussion 23 5. Conclusion 26 6. References 27 7. 국문 초록 32 | - |
dc.format | application/pdf | - |
dc.format.extent | 701758 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | signal regulatory protein alpha | - |
dc.subject | CD47 | - |
dc.subject | macrophage | - |
dc.subject | peptide | - |
dc.subject | graphene nanosheets | - |
dc.subject.ddc | 615 | - |
dc.title | Peptide-coated graphene oxide nanosheets for prolonged blood circulation | - |
dc.title.alternative | 펩타이드 수식 그래핀을 이용한 혈중 지속시간 증강연구 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Gyuthae Park | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 약학대학 약학과 | - |
dc.date.awarded | 2018-02 | - |
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