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The role of α-enolase on the production of interleukin-32 in concanavalin A mediated inflammation and rheumatoid arthritis : Concanavalin A 염증 매개 모델과 류마티스 관절염에서 인터루킨-32 생성과 관련한 알파 에놀레이즈의 역할에 관한 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 강재승 | - |
dc.contributor.author | 이준명 | - |
dc.date.accessioned | 2018-05-29T04:56:27Z | - |
dc.date.available | 2018-05-29T04:56:27Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.other | 000000151427 | - |
dc.identifier.uri | https://hdl.handle.net/10371/142351 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 의과대학 의학과, 2018. 2. 강재승. | - |
dc.description.abstract | Interleukin (IL)-32 is produced by T lymphocytes, natural killer cells, monocytes, and epithelial cells. It has recently been reported that it induces the production of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α, IL-1β, IL-6 and IL-8 and its expression is highly increased in the rheumatoid arthritis (RA) patients. α-Enolase (ENO1) is a glycolytic enzyme and the stimulation of ENO1 induces high level of pro-inflammatory cytokines from concanavalin A (Con A)-activated peripheral blood mononuclear cells (PBMCs) and macrophages from RA patients. In addition, there are many reports that anti-ENO1 antibody is correlated with the disease progression of RA. It implies that ENO1 could regulate IL-32 production during inflammation related with the pathogenesis of RA. Therefore, I performed whether ENO1 is involved in the production of IL-32 underinflammatory condition using Con A-activated PBMCs and RA PBMCs. As a result, it was confirmed by RT-PCR and ELISA that the expression of IL-32 at mRNA and protein levels was increased by stimulation with anti-ENO1 mAb. To investigate the signaling pathway that is stimulated with anti-ENO1 mAb, I did immunoblotting for nuclear factor (NF)-κB and p38 mitogen activated protein kinase (MAPK) pathway. Phosphorylated p65 and p38 MAPK were increased by ENO1 stimulation. Finally, I confirmed that the increased IL-32 production by ENO1 stimulation was inhibited by the pre-treatment of BAY11-7082, SB203580. Taken together, these results suggest that ENO1 plays an important role in inflammation through the induction of IL-32 production that is mediated with the activation of NF-κB and p38 MAPK pathway. | - |
dc.description.tableofcontents | 1. Introduction 1
2. Materials and Methods 4 2.1 Isolation PBMCs 4 2.2 Stimulation of PBMCs with Concanavalin A and anti-ENO1 mAb 4 2.3 Real-time Reverse Transcription-Polymerase Chain Reation (RT-qPCR) 6 2.4 Enzyme-Linked Immunosorbent Assay (ELISA) 6 2.5 Inhibitor study for signalig pathway 6 2.6 Western blot analysis 6 2.7 Statistical analysis 8 3. Results 9 4. Discussion 23 5. References 27 6. Abstract in Korean 34 | - |
dc.format | application/pdf | - |
dc.format.extent | 841079 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | ENO1 | - |
dc.subject | IL-32 | - |
dc.subject | RA | - |
dc.subject | Inflammation | - |
dc.subject.ddc | 610 | - |
dc.title | The role of α-enolase on the production of interleukin-32 in concanavalin A mediated inflammation and rheumatoid arthritis | - |
dc.title.alternative | Concanavalin A 염증 매개 모델과 류마티스 관절염에서 인터루킨-32 생성과 관련한 알파 에놀레이즈의 역할에 관한 연구 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2018-02 | - |
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