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Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer

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dc.contributor.authorYum, Soo-Young-
dc.contributor.authorLee, Song-Jeon-
dc.contributor.authorPark, Sin-Gi-
dc.contributor.authorShin, In-Gang-
dc.contributor.authorHahn, Sang-Eun-
dc.contributor.authorChoi, Woo-Jae-
dc.contributor.authorKim, Hee-Soo-
dc.contributor.authorKim, Hyeong-Jong-
dc.contributor.authorBae, Seong-Hun-
dc.contributor.authorLee, Je-Hyeong-
dc.contributor.authorMoon, Joo-Yeong-
dc.contributor.authorLee, Woo-Sung-
dc.contributor.authorLee, Ji-Hyun-
dc.contributor.authorLee, Choong-Il-
dc.contributor.authorKim, Seong-Jin-
dc.contributor.authorJang, Goo-
dc.date.accessioned2018-07-10T02:09:51Z-
dc.date.available2018-07-10T11:13:10Z-
dc.date.issued2018-05-23-
dc.identifier.citationBMC Genomics, 19(1):387ko_KR
dc.identifier.issn1471-2164-
dc.identifier.urihttps://hdl.handle.net/10371/142726-
dc.description.abstractBackground
Transposon-mediated, non-viral gene delivery is a powerful tool for generating stable cell lines and transgenic animals. However, as multi-copy insertion is the preferred integration pattern, there is the potential for uncontrolled changes in endogenous gene expression and detrimental effects in cells or animals. Our group has previously reported on the generation of several transgenic cattle by using microinjection of the Sleeping Beauty (SB) and PiggyBac (PB) transposons and seeks to explore the long-term effects of this technology on cattle.

Results
Transgenic cattle, one female (SNU-SB-1) and one male (SNU-PB-1), reached over 36months of age with no significant health issues and normal blood parameters. The detection of transgene integration and fluorescent signal in oocytes and sperm suggested the capacity for germline transmission in both of the founder animals. After natural breeding, the founder transgenic cow delivered a male calf and secreted milk containing fluorescent transgenic proteins. The calf expressed green fluorescent protein in primary cells from ear skin, with no significant change in overall genomic stability and blood parameters. Three sites of transgene integration were identified by next-generation sequencing of the calfs genome.

Conclusions
Overall, these data demonstrate that transposon-mediated transgenesis can be applied to cattle without being detrimental to their long-term genomic stability or general health. We further suggest that this technology may be usefully applied in other fields, such as the generation of transgenic animal models.
ko_KR
dc.description.sponsorshipThis study was supported financially by the Research Institute of Veterinary Science, the Creative Veterinary Graduate program of the Brain Korea 21 Program for Leading Universities & Students (BK21 PLUS), the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry
(# 109023–05-5-CG000) and the National Research Foundation of Korea (2017R1A2B3004972). The funding bodies were not involved in the experimental design and data collection, analysis or interpretation of data in this study, nor in the writing of the manuscript.
ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.subjectGermline transmissionko_KR
dc.subjectNext-generation sequencingko_KR
dc.subjectSleeping beautyko_KR
dc.subjectPiggyBacko_KR
dc.subjectTransgenic cattleko_KR
dc.titleLong-term health and germline transmission in transgenic cattle following transposon-mediated gene transferko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor염수영-
dc.contributor.AlternativeAuthor이송전-
dc.contributor.AlternativeAuthor박신기-
dc.contributor.AlternativeAuthor신인강-
dc.contributor.AlternativeAuthor한상은-
dc.contributor.AlternativeAuthor최우재-
dc.contributor.AlternativeAuthor김희수-
dc.contributor.AlternativeAuthor김형종-
dc.contributor.AlternativeAuthor배성훈-
dc.contributor.AlternativeAuthor이제형-
dc.contributor.AlternativeAuthor문주영-
dc.contributor.AlternativeAuthor이우성-
dc.contributor.AlternativeAuthor이지현-
dc.contributor.AlternativeAuthor이충일-
dc.contributor.AlternativeAuthor김성진-
dc.contributor.AlternativeAuthor장구-
dc.identifier.doi10.1186/s12864-018-4760-4-
dc.language.rfc3066en-
dc.rights.holderThe Author(s).-
dc.date.updated2018-05-27T03:36:16Z-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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