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Identification of heterogeneity of colon cancer stem cells by single cell analysis : 단일세포 유전체 분석을 통한 대장암 줄기세포의 이질성 규명
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- Authors
- Advisor
- 김태유
- Major
- 융합과학기술대학원 분자의학 및 바이오제약학과
- Issue Date
- 2018-08
- Publisher
- 서울대학교 대학원
- Description
- 학위논문 (석사)-- 서울대학교 대학원 : 융합과학기술대학원 분자의학 및 바이오제약학과, 2018. 8. 김태유.
- Abstract
- Tumor heterogeneity is one of the ongoing huddles in the field of colon cancer therapy. It is evident that there are countless clones which exhibit different phenotypes and therefore, single cell analysis is inevitable. Cancer stem cells (CSCs) are rare cell population within tumor which is widely known to function in cancer metastasis and recurrence. Although there have been trials to prove intra-tumoral heterogeneity using single cell sequencing, that of CSCs has not been clearly elucidated. Through sequencing of CSCs, one could identify clones in their population with different functions. Here, we articulate the presence of heterogeneity within cancer stem cells through single cell sequencing. As a proof of principle, we performed phenotype-based high-throughput laser isolation and single cell sequencing (PHLI-seq) of 8 CD133 positive cells in a frozen tumor tissue obtained from a patient with colorectal cancer. The result proved that CD133 positive cells were shown to be heterogeneous both in copy number and mutational profiles. . Single cancer stem cell specific mutations such as RNF144A, PAK2, PARP4, ADAM21, HYDIN, KRT38 and CELSR1 could be also detected in metastatic tumor of the same patient. Collectively, these data suggest that single cell analysis used to identify heterogeneity within rare population, will lead to new strategies to tackle colon cancer metastasis.
- Language
- English
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