S-Space College of Natural Sciences (자연과학대학) Dept. of Biological Sciences (생명과학부) Theses (Master's Degree_생명과학부)
Investigation of the cambium regulators for the secondary growth and organogenesis in Raphanus sativus L : 무 뿌리에서 이차 성장 및 기관 재생에 관여하는 형성층 조절자의 규명
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- 자연과학대학 생명과학부
- Issue Date
- 서울대학교 대학원
- 학위논문 (석사)-- 서울대학교 대학원 : 자연과학대학 생명과학부, 2018. 8. 이지영.
- Raphanus sativus or radish is one of the most socioeconomically important root crops in northeast Asia. Radish root develops an edible thick storage root whose growth has been proven to be caused by cell division in the cambium. Although the radish genome has been studied and compared to other members of the Brassicaceae, its broad comprehension is yet to be completed. In this research, we looked at the radish root gene expression during its key development periods, 5, 7 and 9 weeks post seed planting, in two phenotypically different radish lines. We identified conserved cambium regulators as well as new cambium enriched genes that might play a novel function in the vascular cambium. Additionally, we discovered the ability of the radish cambium to reprogram itself to produce adventitious roots (AR). To further understand this process, we investigated how auxin induces AR formation in radish. We found that the auxin-induced WOX11/12 molecular pathway that produces AR in Arabidopsis, also plays an important role in radish AR formation, thus confirming the evolutionary conservation of the pathway. We also analyzed how cambium secondary growth regulators change their expression during AR organogenesis. Interestingly, we found that RsWOX4and RsPXY, key regulators of cambium cell division, change their expression pattern seemingly to aid the vascularization of the AR primordium. This thesis provides an overview of the molecular dynamics in radish taproot cambium, alternative functions of the cambium.
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