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M1 macrophage-derived nanovesicles repolarize M2 macrophages for cancer treatment : 암 치료를 위한 M1 대식세포에서 추출한 나노베지클에 의한 M2 대식세포의 재분화 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김병수 | - |
dc.contributor.author | 추연웅 | - |
dc.date.accessioned | 2018-12-03T01:46:45Z | - |
dc.date.available | 2019-08-02 | - |
dc.date.issued | 2018-08 | - |
dc.identifier.other | 000000152694 | - |
dc.identifier.uri | https://hdl.handle.net/10371/143965 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 공과대학 화학생물공학부, 2018. 8. 김병수. | - |
dc.description.abstract | Cancer immunotherapy is a treatment that activate immune cells to induce anti-tumor immune response. Since macrophages are common immune cells in tumor microenvironment (TME), tumor-associated macrophages (TAM) are studied as a attractive target for cancer immunotherapy. Macrophages are known to have two different phenotypes | - |
dc.description.abstract | M2 macrophages release anti-inflammatory cytokines and angiogenesis factor that potentiate tumor growth. M1 macrophages release pro-inflammatory cytokines and induce anti-tumor immune response. Cancer cells release cytokines that affect TAM to polariae to M2 macrophages. Thus, repolarazation of M2 TAM to M1 macrophages may be promising immunotherapy.
Exosomes are known as nanocarrier that can induce phenotype change in recipient cells. In this study, we used exosome-like nanovesicles derived from M1 macrophages (M1NV) to repolarize M2 macrophages to M1 macrophages. M1NV treatment to M2 macrophage showed successful upregulation of M1 marker mRNA expression, protein expression, and cytokines expression in M2 macrophages. Thus, our study indicates M1NV treatment repolarize M2 TAM to M1 macrophages for cancer immunotherapy. | - |
dc.description.tableofcontents | List of Figures 1
1. Introduction 5 2. Experimental Section 10 2.1 Cell Culture 10 2.2 Preparation of M1NV 12 2.3 Preparations of Exosome 13 2.4 Physicochemical Characterization of M1NV 14 2.5 mRNA Quantification of NV and Cells 15 2.6 MicroRNA Profiling Assay 16 2.7 In vitro Cellular Uptake of M1NV 17 2.8 Viability of Cells after Treatment with M1NV 18 2.9 Transwell Assay 19 2.10 In vitro Analyses of Macrophage Polarization 20 3. Results and Discussion 22 3.1 Characterization of M1NV and Exosomes 22 3.2 In vitro Cellular Uptake and Cytotoxicity of M1NV 27 3.3 Comparison of the Therapeutic Effects between M1NV and M1 Macrophages 30 3.4 M1NV Polarize M2 Macrophages to M1 Type. 34 4. Conclusion 39 5. Reference 40 요약 (국문초록) 46 | - |
dc.format | application/pdf | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject.ddc | 660.6 | - |
dc.title | M1 macrophage-derived nanovesicles repolarize M2 macrophages for cancer treatment | - |
dc.title.alternative | 암 치료를 위한 M1 대식세포에서 추출한 나노베지클에 의한 M2 대식세포의 재분화 연구 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Yeon Woong Choo | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 공과대학 화학생물공학부 | - |
dc.date.awarded | 2018-08 | - |
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