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Regulation of EXLA1 Stability by E3 Ubiquitin Ligase GW2 in Rice
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- Authors
- Advisor
- 서학수
- Major
- 농업생명과학대학 식물생산과학부(작물생명과학전공)
- Issue Date
- 2018-08
- Publisher
- 서울대학교 대학원
- Description
- 학위논문 (석사)-- 서울대학교 대학원 : 농업생명과학대학 식물생산과학부(작물생명과학전공), 2018. 8. 서학수.
- Abstract
- Grain Weight 2 (GW2), known as the E3 ubiquitin ligase, important role in
controlling the size and weight of the grains. Such gene expression and regulation
by GW2 has been studied for a long time. Nevertheless, the post-translational
research of GW2 rarely progressed. Previous studies have confirmed in vitro that
Grain Weight 2 (GW2) in rice could act as an E3 ubiquitin ligase for EXPANSIN
LIKE 1 (EXLA1). Here, we showed that Grain Weight2 (GW2) could regulation
EXLA1 stability. In this paper, particle bombardment experiments showed that
GW2 and EXLA1 interacted in epidermal cells of onion. The yeast two-hybrid
experiment also confirmed that the expansin family except EXLA1 did not bind to
GW2. In addition, the expression of EXLA1 was found to be high in the gw2
mutant and the expression of EXLA1 was found to be different when GA3,
Cytokinin, and Auxin were treated to the WT Norin. We also found that BSG1,
a candidate group other than EXLA1, binds to GW2 through yeast two-hybrid
experiments and in vitro pull down experiments. Taken together, we confirmed that
the stability of EXLA1 was regulated by GW2, and confirmed that it binds to a
candidate group other than EXLA1.
- Language
- English
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