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Synthesis and Biological Characteristics of Low-calorie Omija Juice : 저열량 오미자청 합성과 생물학적 특성 연구

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dc.contributor.advisor김도만-
dc.contributor.author곽소형-
dc.date.accessioned2018-12-03T01:53:06Z-
dc.date.available2020-02-04T00:12:56Z-
dc.date.issued2018-08-
dc.identifier.other000000153122-
dc.identifier.urihttps://hdl.handle.net/10371/144152-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 국제농업기술대학원 국제농업기술학과, 2018. 8. 김도만.-
dc.description.abstractOmija whose binomial name is Schisandra chinensis Turcz. (Baill.) is a medicinal plant that contains many bioactive chemicals called dibenzocyclooctadiene liganans. Schisandrin, Gomisin N, Schizandrin C, Schisandrin A, Gomisins A and G are its major dibenzocyclooctadiene lignans. Using theses rare valuable compounds from omija with sucrose, omija juice was made by two glucansucrases from Leuconostoc mesenteroides B-512FMCM and B-1355CF10. Acceptor reaction occurred by dextransucrase and alternansucrase from respective Leuconostoc mesenteroides until all of sucrose was consumed. More than nine kinds of oligosaccharides which have α-(1,6) linkages as backbones and α-(1,2), α-(1,3), α-(1,4) linkages as branches were synthesized in Omija juice.

Omija oligosaccharides have lowered calories by 61.13% comparing to regular omija juice which have 50% sucrose after treatment with intestinal enzymes.

Newly synthesized omija oligosaccharides reduced formation of the insoluble glucan, mutan which is one of products that causes dental plaque by 96%.

Omija oligosaccharides could give melanogenesis inhibition activity.

This paper revealed omija oligosaccharides have potential for medicinal and cosmetic properties in biological system.
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dc.description.tableofcontentsIntroduction 1

1. Omija 1

1.1. Omija juice. 1

2. Leuconostoc mesenteroides B-512FMCM & B-1355CF10 2

3. Oligosaccharidess 4

3.1. Bioavailability of less-digestible oligosaccharides. 5

4. Staphylococcus mutans and their formation of plaque and dental caries 6

5. Melanogenesis inhibition assay using Zebrafish larvae 7

6. Research purpose of Omija oligosaccharides 8

Materials and Methods 9

1. Preparation of culture media. 9

2. Enzyme preparation from the B-512FMCM & B-1355CF 10strain. 9

3. Fermentation of the B-512FMCM & B-1355CF10 strain. 10

4. Concentration of enzyme from the B-512FMCM & B-1355CF10 strain. 10

5. Preparation of omija oligosaccharides sample. 11

6. Removement of monosaccharides using yeast beads and large-scale production of omija oligosaccharides, omija, and less-digestible oligosaccharides. 12

7. HPLC Analysis of the Less-digestible oligosaccharides 13

8. Prevention of insoluble glucan synthesis by Streptococcus mutans mutansucrase. 13

9. Caloric analysis . 15

10. Melanogenesis inhibition assay using Zebrafish larvae 15

Results 17

1. High-performance liquid chromatography analysis of oligosaccharides 17

2. Thin Layer Chromatography and Maldi-Tof MS analysis in omija oligosaccharides. 19

3. Calorie analysis using D-fructose and D-glucose assay procedure. 21

4. Decrease in insoluble glucan formation from mutansucrase. 23

5. Melanogenesis inhibition activity of Omija and oligosaccharides 25

Discussion 27

Conclusion 29

References 30

Abstract in Korean 36
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dc.formatapplication/pdf-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subject.ddc631-
dc.titleSynthesis and Biological Characteristics of Low-calorie Omija Juice-
dc.title.alternative저열량 오미자청 합성과 생물학적 특성 연구-
dc.typeThesis-
dc.description.degreeMaster-
dc.contributor.affiliation국제농업기술대학원 국제농업기술학과-
dc.date.awarded2018-08-
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