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The antioxidant activity of neutral and acidic polysaccharides from Panax ginseng on Caenorhabditis elegans : 고려인삼에서 추출한 중성 및 산성다당체의 항산화 효과
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 권성원 | - |
| dc.contributor.author | 송얀슈에 | - |
| dc.date.accessioned | 2018-12-03T01:53:50Z | - |
| dc.date.available | 2021-09-23T06:03:58Z | - |
| dc.date.issued | 2018-08 | - |
| dc.identifier.other | 000000151929 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/144171 | - |
| dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 약학대학 약학과, 2018. 8. 권성원. | - |
| dc.description.abstract | Ginseng polysaccharide is a kind of polymer in ginseng. According to the composition, it can be divided into neutral polysaccharide and acidic polysaccharide. It has been reported that, ginseng polysaccharide has lot of pharmacological effects, such as immunomodulation, anti-tumor, anti-oxidant activity, and so on. Because of the large molecular weight and complex structure, many effects have not been fully studied. In this study, we focused on the antioxidant activity comparison between neutral and acidic polysaccharide from Panax ginseng.
A neutral polysaccharide (WGPN) and an acidic polysaccharide (WGPA) were extracted from Panax ginseng by DEAE cellulose ion-exchange chromatography. Their structures were characterized by chemical composition analysis, high performance gel permeation chromatography (HPGPC), Fourier transform infrared spectrometry (FT-IR), and liquid chromatography with diode-array detector (LC-DAD). Further, their antioxidant activities were investigated both in vitro and in vivo. Results showed that WGPA had higher uronic acid content and larger average molecular weight than WGPN. Compared with WGPN, WGPA exhibited higher scavenging activities against ABTS and hydrogen peroxide free radical, higher reducing power and higher metal chelating activity in vitro, as well as preferably inhibitory effect on lipid peroxidation in the mode of Caenorhabditis elegans. | - |
| dc.description.tableofcontents | Contents
Abstract ii List of figures vi List of tables vii 1. Introduction 1 2. Experiment 3 2. 1. Sample collection and pretreatment 3 2. 2. Chemicals and materials 3 2. 2. 1. Chemicals 3 2. 2. 2. Experimental supplies 5 2. 2. 3. Analytical instruments 6 2. 3. Extraction and purification of white ginseng polysaccharide 7 2. 4. Preliminary characterization of WGPN and WGPA 10 2. 4. 1. Chemical properties 10 2. 4. 2. Fourier-transform infrared spectroscopy (FT-IR) 13 2. 4. 3. Monosaccharide composition 13 2. 5. In vitro antioxidant activity assay 15 2. 5. 1. ABTS radical scavenging activity 15 2. 5. 2. Reducing power method 15 2. 5. 3. Metal chelating activity method 16 2. 5. 4. Hydrogen peroxide scavenging activity 16 2. 6. In vivo biological activity assay 17 2. 6. 1. C. elegans strain and culture conditions 17 2. 6. 2. Lethality assay 17 2. 6. 3. Experimental design 18 2. 6. 4. ROS level assay 18 2. 6. 5. Lipid peroxidation assay 18 3. Result and discussion 20 3. 1. Isolation and purification of polysaccharides 20 3. 2. Preliminary characterization 20 3. 2. 1. Chemical compositions and molecular weight of WGPN and WGPA 20 3. 2. 2. FT-IR spectrum 23 3. 2. 3. Monosaccharide composition of WGPN and WGPA 23 3. 3. In vitro antioxidant activities of polysaccharide fractions 24 3. 3. 1. ABTS radical scavenging activity 24 3. 3. 2. Reducing power method 24 3. 3. 3. Metal chelating activity 25 3. 3. 4. Hydrogen peroxide scavenging activity 25 3. 4. In vivo biological activity analysis 28 3. 4. 1. Lethality assay of polysaccharides on C. elegans. 28 3. 4. 2. ROS level assay. 29 3. 4. 3. Lipid peroxidation assay. 29 4. Conclusion 32 5. References 33 국문초록 36 | - |
| dc.format | application/pdf | - |
| dc.format.medium | application/pdf | - |
| dc.language.iso | en | - |
| dc.publisher | 서울대학교 대학원 | - |
| dc.subject.ddc | 615 | - |
| dc.title | The antioxidant activity of neutral and acidic polysaccharides from Panax ginseng on Caenorhabditis elegans | - |
| dc.title.alternative | 고려인삼에서 추출한 중성 및 산성다당체의 항산화 효과 | - |
| dc.type | Thesis | - |
| dc.contributor.AlternativeAuthor | SONG YANXUE | - |
| dc.description.degree | Master | - |
| dc.contributor.affiliation | 약학대학 약학과 | - |
| dc.date.awarded | 2018-08 | - |
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