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Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2-or 3-dimensional environment
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cheong, Jin-Gyu | - |
dc.contributor.author | Song, Dae-Geun | - |
dc.contributor.author | Song, Haeng Eun | - |
dc.contributor.author | Berditchevski, Fedor | - |
dc.contributor.author | Nam, Seo Hee | - |
dc.contributor.author | Jung, Jae Woo | - |
dc.contributor.author | Kim, Hye-Jin | - |
dc.contributor.author | Kim, Ji Eon | - |
dc.contributor.author | Kim, Somi | - |
dc.contributor.author | Ryu, Jihye | - |
dc.contributor.author | Cho, Chang Yun | - |
dc.contributor.author | Lee, Kyung-Min | - |
dc.contributor.author | Lee, Jung Weon | - |
dc.creator | 이정원 | - |
dc.date.accessioned | 2019-04-24T08:28:55Z | - |
dc.date.available | 2020-04-05T08:28:55Z | - |
dc.date.created | 2017-11-15 | - |
dc.date.created | 2017-11-15 | - |
dc.date.issued | 2017-02 | - |
dc.identifier.citation | Oncotarget, Vol.8 No.8, pp.13277-13292 | - |
dc.identifier.issn | 1949-2553 | - |
dc.identifier.uri | https://hdl.handle.net/10371/147836 | - |
dc.description.abstract | The transmembrane 4 L six family proteins TM4SF1, TM4SF4, and TM4SF5 share 40-50% overall sequence identity, but their C-terminus identity is limited. It may be likely that the C-termini of the members are important and unique for own regulatory functions. We thus examined how the TM4SF5 C-terminus affected cellular functions differentially from other family members. Using colon cancer cells expressing wildtype (WT), C-terminus-deleted, or chimeric mutants, diverse cellular functions were explored in 2-dimensional (2D) and 3-dimensional (3D) condition. The C-termini of the proteins were relatively comparable with respect to 2D cell proliferation, although each C-terminal-deletion mutant exhibited increased proliferation relative to the WT. Using chimeric constructs, we found that the TM4SF5 C-terminus was critical for regulating the diverse metastatic functions of TM4SF5, and could positively replace the C-termini of other family members. Replacement of the TM4SF1 or TM4SF4 C-terminus with that of TM4SF5 increased spheroids growth, transwell migration, and invasive dissemination from spheroids in 3D collagen gels. TM4SF5-mediated effects required its extracellular loop 2 linked to the C-terminus via the transmembrane domain 4, with causing c-Src activation. Altogether, the C-terminus of TM4SF5 appears to mediate pro-migratory roles, depending on a structural relay from the second extracellular loop to the C-terminus. | - |
dc.language | 영어 | - |
dc.language.iso | en | en |
dc.publisher | Impact Journals | - |
dc.title | Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2-or 3-dimensional environment | - |
dc.type | Article | - |
dc.identifier.doi | 10.18632/oncotarget.14809 | - |
dc.citation.journaltitle | Oncotarget | - |
dc.identifier.wosid | 000395692000072 | - |
dc.identifier.scopusid | 2-s2.0-85013384895 | - |
dc.description.srnd | OAIID:RECH_ACHV_DSTSH_NO:T201703172 | - |
dc.description.srnd | RECH_ACHV_FG:RR00200001 | - |
dc.description.srnd | ADJUST_YN: | - |
dc.description.srnd | EMP_ID:A078142 | - |
dc.description.srnd | CITE_RATE:5.168 | - |
dc.description.srnd | FILENAME:97JGC-Oncotarget.pdf | - |
dc.description.srnd | DEPT_NM:약학과 | - |
dc.description.srnd | EMAIL:jwl@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | FILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/ca459b0c-ed9a-4250-acc2-5da2cb2e6037/link | - |
dc.citation.endpage | 13292 | - |
dc.citation.number | 8 | - |
dc.citation.startpage | 13277 | - |
dc.citation.volume | 8 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Lee, Jung Weon | - |
dc.identifier.srnd | T201703172 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | SUPERFAMILY MEMBER 4 | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordPlus | TM4SF4 | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordPlus | TETRASPANINS | - |
dc.subject.keywordPlus | MICRODOMAINS | - |
dc.subject.keywordPlus | HEPATOCYTES | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | 3D cell culture | - |
dc.subject.keywordAuthor | migration | - |
dc.subject.keywordAuthor | proliferation | - |
dc.subject.keywordAuthor | spheroids | - |
dc.subject.keywordAuthor | transmembrane 4 L six family | - |
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