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Fabrication of bi-layer scaffold of keratin nanofiber and gelatin-methacrylate hydrogel: Implications for skin graft

Cited 52 time in Web of Science Cited 54 time in Scopus
Authors

Jong Wook Kim; Min Jin Kim; Chang Seok Ki; Hyun Jeong Kim; Young Hwan Park

Issue Date
2017-12
Publisher
Elsevier BV
Citation
International Journal of Biological Macromolecules, Vol.105 No.part 1, pp.541-548
Abstract
Bi-layer scaffold composed of human hair keratin/chitosan nanofiber mat and gelatin methacrylate (GelMA) hydrogel was fabricated by using electrospinning and photopolymerization techniques. To prepare the nanofiber layer, the blend solution of human hair keratin and chitosan (mixture ratio: 5/5) was electrospun using formic acid as a solvent in the presence of poly(ethylene glycol), followed by cross-linking with glutaraldehyde. The tensile strength of the human hair keratin/chitosan nanofiber mat was much higher than that of pure human hair keratin nanofiber mat. Meanwhile, the blend nanofiber mat was relatively more compatible with HaCaT cell proliferation and keratinocyte differentiation than the pure chitosan nanofiber mat. The bi-layer scaffold was prepared by photopolymerization of GelMA under the cross-linked nanofiber mat. To evaluate the feasibility as a skin graft, human fibroblast was encapsulated in the hydrogel layer and HaCaT cells were cultured on the nanofiber layer and they were co-cultured for 10. days. As a result, the encapsulated fibroblasts proliferated in the hydrogel matrix and HaCaT cells formed a cell layer on the top of scaffold, mimicking dermis and epidermis of skin tissue. © 2017 Elsevier B.V.
ISSN
0141-8130
Language
English
URI
https://hdl.handle.net/10371/148151
DOI
https://doi.org/10.1016/j.ijbiomac.2017.07.067
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