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Disruption of endothelial barrier function is linked with hyposecretion and lymphocytic infiltration in salivary glands of Sjogren's syndrome
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cong, Xin | - |
dc.contributor.author | Zhang, Xue Ming | - |
dc.contributor.author | Zhang, Yan | - |
dc.contributor.author | Wei, Tai | - |
dc.contributor.author | He, Qi Hua | - |
dc.contributor.author | Zhang, Li Wei | - |
dc.contributor.author | Hua, Hong | - |
dc.contributor.author | Lee, Sang Woo | - |
dc.contributor.author | Park, Kyungpyo | - |
dc.contributor.author | Yu, Guang Yan | - |
dc.contributor.author | Wu, Li Ling | - |
dc.creator | 박경표 | - |
dc.date.accessioned | 2019-04-25T01:24:15Z | - |
dc.date.available | 2020-04-05T01:24:15Z | - |
dc.date.created | 2019-07-05 | - |
dc.date.created | 2019-07-05 | - |
dc.date.created | 2019-07-05 | - |
dc.date.issued | 2018-10 | - |
dc.identifier.citation | Biochimica et Biophysica Acta - Molecular Basis of Disease, Vol.1864 No.10, pp.3154-3163 | - |
dc.identifier.issn | 0925-4439 | - |
dc.identifier.uri | https://hdl.handle.net/10371/149350 | - |
dc.description.abstract | Sjogren's syndrome (SS) is an inflammatory autoimmune disease that causes hyposecretion in salivary glands. Endothelial tight junctions (TJs) play crucial roles in salivation and barrier function of blood vessels. However, whether the alteration of endothelial TJs were involved in pathogenesis of SS was still unknown. Here, the ultrastructure and function of endothelial TJs in submandibular glands (SMGs) were detected by transmission electron microscopy and in vivo paracellular permeability assay in different aged NOD mouse model for SS. CFSE-labeled lymphocytes were injected into tail vein to trace the infiltration, while claudin-5 expression and distribution were detected by immunofluorescence, qRT-PCR, and western blot. Results showed that the stimulated salivary flow rate was gradually decreased and lymphocytic infiltration was found as age increased in 12- and 21-week-old NOD mice, but not 7-week-old NOD mice. Blood vessels were dilated, while endothelial TJ width and paracellular tracer transport were increased in 12-week-old NOD mice. Moreover, the injected CFSE-labeled lymphocytes were observed in SMGs of 12-week-old NOD mice. Claudin-5 level was increased and relocalized from the apical portion of neighboring endothelial cells to lateral membranes and cytoplasm in 12-week-old NOD mice. Additionally, the alteration of claudin-5 expression and distribution was further confirmed in labial salivary glands and bilateral parotid glands from SS patients. In cultured human microvessel endothelial cell line (HMEC-1), IFN-gamma stimulation significantly increased claudin-5 expression. Taken together, we identified that the endothelial TJ barrier was disrupted and contributed to the development of salivary hyposecretion and lymphocytic infiltration in SS. | - |
dc.language | 영어 | - |
dc.language.iso | en | en |
dc.publisher | Elsevier BV | - |
dc.title | Disruption of endothelial barrier function is linked with hyposecretion and lymphocytic infiltration in salivary glands of Sjogren's syndrome | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbadis.2018.07.002 | - |
dc.citation.journaltitle | Biochimica et Biophysica Acta - Molecular Basis of Disease | - |
dc.identifier.wosid | 000445312000005 | - |
dc.identifier.scopusid | 2-s2.0-85050114854 | - |
dc.description.srnd | OAIID:RECH_ACHV_DSTSH_NO:T201809346 | - |
dc.description.srnd | RECH_ACHV_FG:RR00200001 | - |
dc.description.srnd | ADJUST_YN: | - |
dc.description.srnd | EMP_ID:A001677 | - |
dc.description.srnd | CITE_RATE:5.108 | - |
dc.description.srnd | DEPT_NM:치의과학과 | - |
dc.description.srnd | EMAIL:kppark@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.citation.endpage | 3163 | - |
dc.citation.number | 10 | - |
dc.citation.startpage | 3154 | - |
dc.citation.volume | 1864 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Lee, Sang Woo | - |
dc.contributor.affiliatedAuthor | Park, Kyungpyo | - |
dc.identifier.srnd | T201809346 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | TIGHT JUNCTION PROTEINS | - |
dc.subject.keywordPlus | BLOOD-FLOW | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | PERMEABILITY | - |
dc.subject.keywordPlus | DYSFUNCTION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | CLAUDIN-5 | - |
dc.subject.keywordPlus | OCCLUDIN | - |
dc.subject.keywordPlus | FLUID | - |
dc.subject.keywordAuthor | Claudin-5 | - |
dc.subject.keywordAuthor | Endothelium | - |
dc.subject.keywordAuthor | Salivary gland | - |
dc.subject.keywordAuthor | Sjogren&apos | - |
dc.subject.keywordAuthor | s syndrome | - |
dc.subject.keywordAuthor | Tight junction | - |
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