Browse

Identification of potential biomarkers for diagnosis of pancreatic and biliary tract cancers by sequencing of serum microRNAs

Cited 4 time in Web of Science Cited 5 time in Scopus
Authors
Kim, Kwondo; Yoo, DongAhn; Lee, Hee Seung; Lee, Kyong Joo; Park, Soo Been; Kim, Chanyang; Jo, Jung Hyun; Jung, Dawoon E.; Song, Si Young
Issue Date
2019-05-16
Publisher
BioMed Central
Citation
BMC Medical Genomics. 12(1):62
Keywords
Serum miRNAPancreatic cancerBiliary tract cancerDiagnosisBiomarker
Abstract
Background
Pancreatic and biliary tract cancer (PC and BTC, respectively) are difficult to diagnose because of their clinical characteristics; however, recent studies suggest that serum microRNAs (miRNAs) might be the key to developing more efficient diagnostic methods for these cancers.

Methods
We analysed the genome-wide expression of serum miRNAs in PC and BTC patients to identify novel biomarker candidates using high-throughput sequencing and experimentally validated miRNAs on clinical samples.

Results
Statistical and classification analysis of the serum miRNA-expression profiles of 55 patient samples showed distinguishable patterns between cancer patients and healthy controls; however, we were unable to distinguish the two cancers. We found that three of the highest performing miRNAs were capable of distinguishing cancer patients from controls, with an accuracy of 92.7%. Additionally, dysregulation of these three cancer-specific miRNAs was demonstrated in an independent sample group by quantitative reverse transcription polymerase chain reaction.

Conclusions
These results suggested three candidate serum miRNAs (mir-744-5p, mir-409-3p, and mir-128-3p) as potential biomarkers for PC and BTC diagnosis.
ISSN
1755-8794
Language
English
URI
http://hdl.handle.net/10371/153937
DOI
https://doi.org/10.1186/s12920-019-0521-8
Files in This Item:
Appears in Collections:
College of Natural Sciences (자연과학대학)Program in Bioinformatics (협동과정-생물정보학전공)Journal Papers (저널논문_협동과정-생물정보학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse