S-Space College of Natural Sciences (자연과학대학) Program in Bioinformatics (협동과정-생물정보학전공) Journal Papers (저널논문_협동과정-생물정보학전공)
Identification of potential biomarkers for diagnosis of pancreatic and biliary tract cancers by sequencing of serum microRNAs
- Kim, Kwondo; Yoo, DongAhn; Lee, Hee Seung; Lee, Kyong Joo; Park, Soo Been; Kim, Chanyang; Jo, Jung Hyun; Jung, Dawoon E.; Song, Si Young
- Issue Date
- BioMed Central
- BMC Medical Genomics. 12(1):62
Pancreatic and biliary tract cancer (PC and BTC, respectively) are difficult to diagnose because of their clinical characteristics; however, recent studies suggest that serum microRNAs (miRNAs) might be the key to developing more efficient diagnostic methods for these cancers.
We analysed the genome-wide expression of serum miRNAs in PC and BTC patients to identify novel biomarker candidates using high-throughput sequencing and experimentally validated miRNAs on clinical samples.
Statistical and classification analysis of the serum miRNA-expression profiles of 55 patient samples showed distinguishable patterns between cancer patients and healthy controls; however, we were unable to distinguish the two cancers. We found that three of the highest performing miRNAs were capable of distinguishing cancer patients from controls, with an accuracy of 92.7%. Additionally, dysregulation of these three cancer-specific miRNAs was demonstrated in an independent sample group by quantitative reverse transcription polymerase chain reaction.
These results suggested three candidate serum miRNAs (mir-744-5p, mir-409-3p, and mir-128-3p) as potential biomarkers for PC and BTC diagnosis.