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In Situ Subcellular Detachment of Cells Using a Cell-Friendly Photoresist and Spatially Modulated Light

Cited 7 time in Web of Science Cited 6 time in Scopus
Authors

Park, Jeehun; Kim, Taeyup; Choi, Jong Chul; Doh, Junsang

Issue Date
2019-07
Publisher
Wiley-VCH Verlag
Citation
Advanced Science, Vol.6 No.14, p. 1900566
Abstract
Dynamic adhesion and detachment of subcellular regions occur during cell migration, thus a technique allowing precise control of subcellular detachment of cells will be useful for cell migration study. Previous methods for cell detachment were developed either for harvesting cells or cell sheets attached on surfaces with low resolution patterning capability, or for detaching subcellular regions located on predefined electrodes. In this paper, a method that allows in situ subcellular detachment of cells with approximate to 1.5 mu m critical feature size while observing cells under a fluorescence microscope is introduced using a cell-friendly photoresist and spatially modulated light. Using this method, a single cell, regions in cell sheets, and a single focal adhesion complex within a cell are successfully detached. Furthermore, different subcellular regions of migrating cells are detached and changes in cell polarity and migration direction are quantitatively analyzed. This method will be useful for many applications in cell detachment, in particular when subcellular resolution is required.
ISSN
2198-3844
Language
ENG
URI
https://hdl.handle.net/10371/154218
DOI
https://doi.org/10.1002/advs.201900566
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