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Src kinase is a novel target for anti-skin inflammation of apigenin
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이형주 | - |
dc.contributor.author | 박지만 | - |
dc.date.accessioned | 2019-06-25T15:34:09Z | - |
dc.date.available | 2019-06-25T15:34:09Z | - |
dc.date.issued | 2012-02 | - |
dc.identifier.other | 000000000922 | - |
dc.identifier.uri | https://hdl.handle.net/10371/154815 | - |
dc.identifier.uri | http://dcollection.snu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000000922 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부(바이오모듈레이션전공), 2012. 2. 이형주. | - |
dc.description.abstract | Apigenin, a flavonoid abundant in various vegetables and fruits including guava, parsley, and onion, has been reported to possess anti-carcinogenic effects. However, the chemopreventive molecular target of apigenin on UV-induced skin inflammation has not been fully understood. Herein, I examined the anti-inflammatory mechanism of apigenin in JB6 P+ mouse epidermal cells and SKH-1 hairless mice. Apigenin at 10 to 40 μM suppressed UVB (0.05 J/cm2)-induced expression of cyclooxygenase-2 (COX-2) and activation of activator protein-1 (AP-1) and nuclear factor-κB (NF-κB) in JB6 P+ cells. Immunoblot and kinase activity assay showed that apigenin at 10 to 40 μM attenuated Src kinase activity and subsequently inhibited UVB-induced phosphorylation of mitogen-activated protein kinase (MAPK) and Akt-involved signaling molecules. Pull-down assay revealed that apigenin binds with Src in an ATP-competitive manner. In vivo experiment revealed that topical application of apigenin at 7.5 and 30 nmol significantly inhibits UVB (0.18 J/cm2)-induced ear edema development in SKH-1 hairless mice. I also found that topical application of apigenin at 30 and 120 nmol attenuated COX-2 expression and Src kinase activation in the UVB (0.18 J/cm2)-irradiated skin of SKH-1 hairless mice. Taken together, these findings suggest that apigenin exerts potent chemopreventive activity against UVB-induced skin inflammation mainly by targeting Src. | - |
dc.format.extent | 43 | - |
dc.language.iso | eng | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject.ddc | 571 | - |
dc.title | Src kinase is a novel target for anti-skin inflammation of apigenin | - |
dc.type | Thesis | - |
dc.type | Dissertation | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 농생명공학부(바이오모듈레이션전공) | - |
dc.date.awarded | 2012-02 | - |
dc.identifier.holdings | 000000000006▲000000000011▲000000000922▲ | - |
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