Browse

AKAP12 regulates the blood-brain barrier permeability by modulating the tight junction in zebrafish

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
장유진
Advisor
김규원
Major
분자의학 및 바이오제약학과
Issue Date
2012-02
Publisher
서울대학교 대학원
Description
학위논문 (석사)-- 서울대학교 대학원 : 분자의학 및 바이오제약학과, 2012. 2. 김규원.
Abstract
The Blood-Brain Barrier(BBB) has a crucial role in maintaining central nervous system(CNS) environments and protecting the CNS from pathogens and other harmful substances. The tight junction is the major component of maintaining the permeability of the BBB and is established with tight junction proteins between endothelial cells. Previous study reported that zebrafish(Danio rerio) also has the BBB and it starts to develop 3 days post fertilization(dpf). AKAP12 is a scaffolding protein and it has various roles in regulating cell migration, involving the BBB formation and suppressing tumors.
To investigate whether the AKAP12 regulates the BBB development in zebrafish, expression of AKAP12 was knock-downed in zebrafish embryos by morpholinos injection. The AKAP12 knockdown zebrafish has shown hemorrhage in head region(3dpf). In addition, fluorescent dye was injected into common cardinal vein, which also has shown leakage in brain micro-vessels. Therefore, it was assumed that AKAP12 could be involved in the expression and distribution of tight junction proteins (Claudin-5, ZO-1). To identify this suggestion, the expression of tight junction proteins was examined in the mRNA and protein level. As a result, it has shown that mRNA expression of both Claudin-5 and ZO-1 was not changed but protein expression was decreased. Taken together, it was suggested that AKAP12 is involved in regulating the BBB permeability by modulating the expressions of tight junction proteins in zebrafish.
Language
eng
URI
https://hdl.handle.net/10371/154952

http://dcollection.snu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000000903
Files in This Item:
There are no files associated with this item.
Appears in Collections:
Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Theses (Master's Degree_분자의학 및 바이오제약학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse